产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
MDH1, 1-334aa, 人类, 多聚组氨酸标签, 大肠杆菌
目录 :
MBS203418
规格 :
0.1毫克
价格 :
310美元
更多信息或购买 :
产品信息
目录号 :
MBS203418
产品类型 :
重组蛋白
产品全称 :
MDH1, 1-334aa, 人类, 多聚组氨酸标签, 大肠杆菌
产品简称 :
MDH1
产品名称同义词 :
MDH-s; MDHA; MOR2; Malate dehydrogenase; cytoplasmic
其他名称 :
malate dehydrogenase, cytoplasmic isoform 2; Malate dehydrogenase, cytoplasmic; malate dehydrogenase, cytoplasmic; cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32; soluble malate dehydrogenase; malate dehydrogenase 1, NAD (soluble); Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96)
产品基因名称 :
MDH1
其他基因名称 :
MDH1;MDH1;MDHA;MOR2;MDH-s;HEL-S-32;MGC:1375;MDHA
UniProt数据库进入名 :
MDHC_HUMAN
序列长度 :
334
序列 :
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH
纯度 :
> 95% by SDS - PAGE
形式 :
液体。 在20 mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油
浓度 :
1毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Human. Tag: His-tag
其他信息2 :
Expression System: E Coli. Bioactivity: Specific activity is > 100 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25C. Assay: 1. Prepare a 1.45 ml assay buffer. The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid. 2. Add 50 ul of recombinant MDH1 protein with various concentrations (0.03 ug, 0.06 ug, 0.125 ug) in assay buffer. 3. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.
产品种类 :
Cancer
产品描述 :
MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
产品引用 :
Youn HD., et al. (2009) Cell Death Differ. 16(5):738-48. Waye MM., et al (2005) J Cell Biochem. 94(4):763-73.
NCBI GI登录号 :
5174539
NCBI登录号 :
NP_005908
NCBI基因登录号 :
NM_005917.3
NCBI分子量 :
37.4 kDa(342aa), confirmed by MALDI-TOF
NCBI信号通路 :
Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421)
NCBI总结 :
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]
UniProt数据库总结 :
MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]. Protein type: EC 1.1.1.96; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glyoxylate and dicarboxylate. Chromosomal Location of Human Ortholog: 2p13.3. Cellular Component: centrosome; extracellular space; mitochondrion; cytoplasm; cytosol. Molecular Function: malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding. Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis
尺寸1 :
0.1毫克
价格1 :
310美元
尺寸2 :
0.5毫克
价格2 :
720
更多信息或购买 :
公司信息
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MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。