产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
增殖细胞核抗原抗体(克隆k1A3)
目录 :
MBS200091
规格 :
0.05毫升
价格 :
240美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
k1A3
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 酶免疫法
更多信息或购买 :
产品信息
目录号 :
MBS200091
产品类型 :
抗体
产品全称 :
增殖细胞核抗原抗体(克隆k1A3)
产品简称 :
增殖细胞核抗原
产品名称同义词 :
MGC8367;增殖细胞核抗原;增殖细胞核抗原;增殖细胞核抗原
其他名称 :
proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; DNA polymerase delta auxiliary protein; cyclin; proliferating cell nuclear antigen; Cyclin
产品基因名称 :
增殖细胞核抗原
其他基因名称 :
增殖细胞核抗原;增殖细胞核抗原;ATLD2;增殖细胞核抗原
UniProt数据库进入名 :
PCNA_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG3, k
克隆 :
k1A3
宿主 :
小鼠
反应物种 :
人类
序列长度 :
261
纯度 :
By protein-G affinity chromatography
形式 :
液体。 用品在磷酸盐缓冲液(pH值7.4)含0.1%叠氮化钠
浓度 :
1毫克/毫升
储存稳定性 :
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
ELISA (EIA), Western Blot (WB) (Cell lysate)
应用笔记 :
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
其它信息1 :
Antigen Species: Human. Clone: Anti-human PCNA mAb, clone k1A3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PCNA protein. Immunogen: Recombinant human PCNA (1-261aa) purified from E Coli
产品种类 :
细胞周期
产品描述 :
PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.
产品引用 :
Moldovan GL, et al., (2007) Cell 18; 129(4):665-679 Matsumoto k, et al., (1987) EMBO 6(3):637-642
NCBI GI登录号 :
4505641
NCBI登录号 :
NP_002583
NCBI基因登录号 :
NM_002592.2
NCBI分子量 :
28,769 Da
NCBI信号通路 :
BARD1 Signaling Events Pathway (137959); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (890555); Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054)
NCBI总结 :
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3 - 5 exonuclease and 3 -phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Protein type: DNA replication; Cell cycle regulation. Chromosomal Location of Human Ortholog: 20pter-p12. Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; DNA replication factor C complex; nuclear replication fork; nucleus. Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding. Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; leading strand elongation; DNA repair; response to lipid; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle. Disease: Ataxia-telangiectasia-like Disorder 2
尺寸1 :
0.05毫升
价格1 :
240美元
尺寸2 :
0.1毫升
价格2 :
315
更多信息或购买 :
公司信息
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MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。