ELISA/assay

人类胰岛素受体抗体酶联免疫吸附试剂盒

MBS006475

48-Strip-Wells

435美元

酶联免疫吸附试剂盒

人类胰岛素受体抗体酶联免疫吸附试剂盒

胰岛素受体抗体

胰岛素受体;胰岛素受体;胰岛素受体;IR;胰岛素受体

AIRA

INSR;INSR;HHF5;CD220;IR

人类

1382

Store all reagents at 2-8 degree C

Samples: Serum, plasma, tissue homogenate, cell culture supernates and other biological fluids. Assay Type: Sandwich

Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (?) are less than 15%. Intended Uses: For the qualitative determination of activated Human Insulin Receptor Antibody (AIRA) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.

Principle of the assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human AIRA in samples. Add sample to wells pre-coated with one Human Insulin Receptor antigen, at same time add HRP-conjugated Human Insulin Receptor antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human AIRA in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.

308153655

P06213.4

AGE/RAGE Pathway (698754); AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); DNA Damage Response (only ATM Dependent) Pathway (198827); FoxO Signaling Pathway (921162)

After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

INSR: a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). The holoenzyme is cleaved into two chains, the alpha and beta subunits. The active complex is a tetramer containing 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains constitute the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Familial mutations associated with insulin resistant diabetes, acanthosis nigricans, pineal hyperplasia, and polycystic ovary syndrome. SNP variants may be associated with polycystic ovary syndrome, atypical migraine and diabetic hyperlipidemia. Mutations also cause leprechaunism, a severe insulin resistance syndrome causing growth retardation and death in early infancy. Two isoforms of the human protein are produced by alternative splicing. The Short isoform has a higher affinity for insulin than the longer. Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta. Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas. Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; Membrane protein, integral; TK group; InsR family. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; endosome membrane; caveola; receptor complex. Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; insulin-like growth factor I binding; GTP binding; insulin receptor substrate binding; protein-tyrosine kinase activity; PTB domain binding; phosphoinositide 3-kinase binding; receptor signaling protein tyrosine kinase activity; insulin-like growth factor II binding; ATP binding; insulin receptor activity. Biological Process: heart morphogenesis; epidermis development; positive regulation of nitric oxide biosynthetic process; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; regulation of embryonic development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; glucose homeostasis; positive regulation of glucose import; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; male sex determination; positive regulation of cell proliferation; protein heterotetramerization; positive regulation of developmental growth; positive regulation of mitosis; activation of protein kinase B; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular response to insulin stimulus; carbohydrate metabolic process; positive regulation of glycolysis; activation of protein kinase activity; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; positive regulation of DNA replication; positive regulation of cell migration; transformation of host cell by virus. Disease: Diabetes Mellitus, Insulin-resistant, With Acanthosis Nigricans; Hyperinsulinemic Hypoglycemia, Familial, 5; Pineal Hyperplasia, Insulin-resistant Diabetes Mellitus, And Somatic Abnormalities; Donohue Syndrome

48-Strip-Wells

435美元

96-Strip-Wells

600