产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSF1多克隆抗体
目录 :
PA3-017
规格 :
100微升
价格 :
美国430.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 2
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图像
图像 1 :
Western blot analysis of Heat Shock Factor 1 (HSF1) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSF1 rabbit polyclonal antibody (Product # PA3-017) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
图像 2 :
Immunoprecipitation of Heat Shock Factor 1 (HSF 1) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500µg whole cell lysate with 2µg of HSF1 polyclonal antibody (Product # PA3-017) overnight on a rocking platform at 4°C. Immune complexes were captured on 50µl Protein A/G Plus Agarose (Product #20423), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSF1 polyclonal antibody (Product # PA3-017) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean Blot IP Detection Reagent-HRP (Product # 21232) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
图像 3 :
Immunofluorescent analysis of Heat Shock Factor 1 (HSF1) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSF1 polyclonal antibody (Product # PA3-017), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
产品信息
产品类型 :
抗体
产品名称 :
HSF1多克隆抗体
目录# :
PA3-017
规格 :
100微升
价格 :
美国430.00
克隆性 :
多克隆
宿主 :
兔
反应物种 :
C. elegans, Human, Mouse
应用 :
ELISA: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:50, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:10,000
物种 :
C. elegans, Human, Mouse
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
免疫原 :
重组人类HSF1在大肠杆菌中表达
格式 :
液体
应用w/稀释 :
ELISA: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:50, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:10,000
别名 :
AA960185; heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF; HSF 1; Hsf1; Hsf1alpha; Hsf1beta; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; HSTF1
更多信息或购买 :
公司信息
赛默飞世尔