产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Phospho-Huntingtin (Ser421) Polyclonal Antibody
目录 :
PA1-087
规格 :
100微升
价格 :
美国500
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Phospho-Htt (pSer421) was performed on 293T cell lysates from cells transfected with full-length Htt (Lanes 1 and 2) or an Htt Ser421 mutant (Lanes 3 and 4). Following transfection, cells were left untreated (Lanes 1 and 3) or treated with PPI inhibitor (Lanes 2 and 4) to increase phosphorylation of Htt at Ser421. Lysates were loaded onto a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Phospho-Htt (pSer421) polyclonal antibody (Product # PA1-087) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:30,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
产品信息
产品类型 :
抗体
产品名称 :
Phospho-Huntingtin (Ser421) Polyclonal Antibody
目录# :
PA1-087
规格 :
100微升
价格 :
美国500
克隆性 :
多克隆
纯度 :
亲和色谱
宿主 :
兔
反应物种 :
人类, 小鼠
应用 :
Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry: 1:100 - 1:200, Western Blot: 1:500
物种 :
人类, 小鼠
抗体亚型 :
IgG
储存 :
-20°C
描述 :
Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
免疫原 :
Synthetic phosphopeptide conjugated to KLH corresponding to residues GGRSRSG(pS)IVELI (414-426) of Human HTT
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry: 1:100 - 1:200, Western Blot: 1:500
别名 :
AI256365; C430023I11Rik; Hd; HD protein; HD protein homolog; Hdh; HTT; huntingtin; huntingtin (Huntington disease); Huntingtin, myristoylated N-terminal fragment; Huntington disease gene homolog; huntington disease protein; huntington disease protein homolog; IT15; SLC6A4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
更多信息或购买 :
公司信息
赛默飞世尔