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抗体

HSF1单克隆抗体(10H8)

MA5-27688

100微克

美国476.00

单克隆

大鼠

未共轭

10H8

豚鼠, 中国人仓鼠, 人类, 小鼠, 大鼠, 牛, domestic rabbit, 猕猴

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀

HSF1单克隆抗体(10H8)

MA5-27688

100微克

美国476.00

单克隆

蛋白质G

大鼠

牛, 豚鼠, 仓鼠, 人类, 小鼠, 兔, 大鼠, 猕猴

ELISA: Assay-Dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (PFA fixed): 1:1,000, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000

牛, 豚鼠, 仓鼠, 人类, 小鼠, 兔, 大鼠, 猕猴

10H8

IgG1

-20°C

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

液体

ELISA: Assay-Dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (PFA fixed): 1:1,000, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000

AA960185; heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF; HSF 1; Hsf1; Hsf1alpha; Hsf1beta; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; HSTF1