产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
ARF1/ARF3/ARF5/ARF6单克隆抗体(1D9)
目录 :
MA3-060
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
1D9
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 21
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
| |
| Kumari S, Mayor S. ARF1 is directly involved in dynamin-independent endocytosis. Nat Cell Biol. 2008;10:30-41 pubmed
|
| Belov G, Habbersett C, Franco D, Ehrenfeld E. Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication. J Virol. 2007;81:9259-67 pubmed
|
| Shen X, Xu K, Fan Q, Pacheco Rodriguez G, Moss J, Vaughan M. Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake. Proc Natl Acad Sci U S A. 2006;103:2635-40 pubmed
|
| Choi W, Karim Z, Whiteheart S. Arf6 plays an early role in platelet activation by collagen and convulxin. Blood. 2006;107:3145-52 pubmed
|
| Belov G, Fogg M, Ehrenfeld E. Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor. J Virol. 2005;79:7207-16 pubmed
|
| Dell Angelica E, Puertollano R, Mullins C, Aguilar R, Vargas J, Hartnell L, et al. GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. J Cell Biol. 2000;149:81-94 pubmed
|
Wang J, Sun H, Macia E, Kirchhausen T, Watson H, Bonifacino J, et al. PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal. Mol Biol Cell. 2007;18:2646-55 pubmed
| |
Hashimoto S, Onodera Y, Hashimoto A, Tanaka M, Hamaguchi M, Yamada A, et al. Requirement for Arf6 in breast cancer invasive activities. Proc Natl Acad Sci U S A. 2004;101:6647-52 pubmed
| |
Chan D, Strang M, Judson B, Brown W. Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes. Mol Biol Cell. 2004;15:1871-80 pubmed
| |
Spitzenberger F, Pietropaolo S, Verkade P, Habermann B, Lacas Gervais S, Mziaut H, et al. Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells. J Biol Chem. 2003;278:26166-73 pubmed
| |
Pullikuth A, Weidman P. In vitro transport on cis and trans sides of the Golgi involves two distinct types of coatomer and ADP-ribosylation factor-independent transport intermediates. J Biol Chem. 2002;277:50355-64 pubmed
| |
Lu L, Horstmann H, Ng C, Hong W. Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J Cell Sci. 2001;114:4543-55 pubmed
| |
Ahluwalia J, Topp J, Weirather K, Zimmerman M, Stamnes M. A role for calcium in stabilizing transport vesicle coats. J Biol Chem. 2001;276:34148-55 pubmed
| |
Mazaki Y, Hashimoto S, Okawa K, Tsubouchi A, Nakamura K, Yagi R, et al. An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization. Mol Biol Cell. 2001;12:645-62 pubmed
| |
Ooi C, Dell Angelica E, Bonifacino J. ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes. J Cell Biol. 1998;142:391-402 pubmed
| |
Happe S, Weidman P. Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent. J Cell Biol. 1998;140:511-23 pubmed
| |
Cavenagh M, Whitney J, Carroll K, Zhang C, Boman A, Rosenwald A, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J Biol Chem. 1996;271:21767-74 pubmed
| |
Serafini T, Orci L, Amherdt M, Brunner M, Kahn R, Rothman J. ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell. 1991;67:239-53 pubmed
| |
Zeuzem S, Feick P, Zimmermann P, Haase W, Kahn R, Schulz I. Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes. Proc Natl Acad Sci U S A. 1992;89:6619-23 pubmed
|
图像
图像 1 :
Immunofluorescent analysis of ADP-Ribosylation Factor using ADP-Ribosylation Factor Monoclonal Antibody (1D9) (Product# MA3-060 ) shows staining in MCF-7 Cells. ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing ADP-Ribosylation Factor (Product# MA3-060 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
ARF1/ARF3/ARF5/ARF6单克隆抗体(1D9)
目录# :
MA3-060
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
狗, 仓鼠, 人类, 小鼠, 大鼠
应用 :
Flow Cytometry: 1/20, Immunocytochemistry: 1:100, Immunohistochemistry: 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:3000
物种 :
狗, 仓鼠, 人类, 小鼠, 大鼠
克隆 :
1D9
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
免疫原 :
重组人类ARF1
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1/20, Immunocytochemistry: 1:100, Immunohistochemistry: 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:3000
别名 :
5430400P17Rik; AA407803; Ac1-253; ADP ribosylation factor 1; ADP ribosylation factor 3; ADP ribosylation factor 4; ADP ribosylation factor 5; ADP ribosylation factor 6; ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; AI788669; AI854770; ARF; Arf1; Arf2; ARF3; ARF4; Arf5; ARF6; AW496366; I79_003112; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein
更多信息或购买 :
公司信息
赛默飞世尔