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抗体

DYKDDDDK标签单克隆抗体(FG4R), DyLight系列染料488

MA1-91878-D488

50微升

美国225.00

单克隆

小鼠

DyLight488

FG4R

免疫印迹, 免疫细胞化学

DYKDDDDK标签单克隆抗体(FG4R), DyLight系列染料488

MA1-91878-D488

50微升

美国225.00

单克隆

蛋白质A

小鼠

标签

Immunocytochemistry: 5 µg/mL, Western Blot: 1:1,000

标签

FG4R

IgG2b

4° C, 不冻结

DYKDDDDK Tag (FLAG® tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. FLAG is a trademark of Sigma-Aldrich Biotechnology LP

Synthetic peptide (DYKDDDDK) coupled to KLH.

液体

Immunocytochemistry: 5 µg/mL, Western Blot: 1:1,000

DDDDK;DDDDK表位标签;ECS表位标签;ECS标签;FLAG标签;FLAG标签标签