产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Presenilin 1 Monoclonal Antibody (APS 11)
目录 :
MA1-751
规格 :
200微克
价格 :
美国450.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
APS 11
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
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文章摘录数: 1
| 参考文献 |
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Diehlmann A, Ida N, Weggen S, Grunberg J, Haass C, Masters C, et al. Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies. J Neurosci Res. 1999;56:405-19 pubmed
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图像
图像 1 :
图像 2 :
Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
图像 3 :
Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
Presenilin 1 Monoclonal Antibody (APS 11)
目录# :
MA1-751
规格 :
200微克
价格 :
美国450.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
ELISA: 4.5 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 0.75 µg/mL, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
物种 :
Human, Mouse, Non-human primate, Rat
克隆 :
APS 11
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.
免疫原 :
合成多肽对应于氨基酸残基C H(21)L S N T V R S Q N D N R E(34)人的PS1
格式 :
液体
应用w/稀释 :
ELISA: 4.5 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 0.75 µg/mL, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
别名 :
AD3; Ad3h; Alzheimer Disease 3; FAD; Minilin; presenilin 1; presenilin 1 (Alzheimer disease 3); presenilin1; presenilin-1; Presenilin-1 CTF subunit; Presenilin-1 CTF12; Presenilin-1 NTF subunit; Protein S182; PS1; PS-1; PS1-CTF12; PSEN1; Psnl1; S182; S182 protein; Senilin 1
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公司信息
赛默飞世尔