产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
糖皮质激素受体单克隆抗体(BuGR2)
目录 :
MA1-510
规格 :
100微克
价格 :
美国446.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
BuGR2
反应物种 :
豚鼠, African green monkey, fission yeast, 人类, 小鼠, 大鼠, 家羊, domestic rabbit
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, EMSA, 染色质免疫沉淀
, 免疫组化-石蜡切片, 免疫组化-冰冻切片, 免疫组化基因敲除验证
更多信息或购买 :
文章摘录数: 172
出版应用/物种/样本/稀释 | 参考文献 |
---|---|
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| Arambasić J, Poznanovic G, Ivanovic Matic S, Bogojevic D, Mihailovic M, Uskoković A, et al. Association of the glucocorticoid receptor with STAT3, C/EBPbeta, and the hormone-responsive element within the rat haptoglobin gene promoter during the acute phase response. IUBMB Life. 2010;62:227-36 pubmed 出版商
|
| |
| |
| |
| |
| |
| |
| |
| |
| Vujcić M, Velickovic N, Ruzdijic S. Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. Comp Biochem Physiol B Biochem Mol Biol. 2007;148:463-9 pubmed
|
| Wang D, Wang Q, Awasthi S, Simons S. Amino-terminal domain of TIF2 is involved in competing for corepressor binding to glucocorticoid and progesterone receptors. Biochemistry. 2007;46:8036-49 pubmed
|
| Antonow Schlorke I, Muller T, Brodhun M, Wicher C, Schubert H, Nathanielsz P, et al. Betamethasone-related acute alterations of microtubule-associated proteins in the fetal sheep brain are reversible and independent of age during the last one-third of gestation. Am J Obstet Gynecol. 2007;196:553.e1-6 pubmed
|
| Morsink M, Joels M, Sarabdjitsingh R, Meijer O, de Kloet E, Datson N. The dynamic pattern of glucocorticoid receptor-mediated transcriptional responses in neuronal PC12 cells. J Neurochem. 2006;99:1282-98 pubmed
|
| Presman D, Hoijman E, Ceballos N, Galigniana M, Pecci A. Melatonin inhibits glucocorticoid receptor nuclear translocation in mouse thymocytes. Endocrinology. 2006;147:5452-9 pubmed
|
| Erlacher M, Knoflach M, Stec I, Böck G, Wick G, Wiegers G. TCR signaling inhibits glucocorticoid-induced apoptosis in murine thymocytes depending on the stage of development. Eur J Immunol. 2005;35:3287-96 pubmed
|
| Virgolini M, Chen K, Weston D, Bauter M, Cory Slechta D. Interactions of chronic lead exposure and intermittent stress: consequences for brain catecholamine systems and associated behaviors and HPA axis function. Toxicol Sci. 2005;87:469-82 pubmed
|
| Webster J, Sternberg E. Anthrax lethal toxin represses glucocorticoid receptor (GR) transactivation by inhibiting GR-DNA binding in vivo. Mol Cell Endocrinol. 2005;241:21-31 pubmed
|
Hawle P, Siepmann M, Harst A, Siderius M, Reusch H, Obermann W. The middle domain of Hsp90 acts as a discriminator between different types of client proteins. Mol Cell Biol. 2006;26:8385-95 pubmed
| |
Heitzer M, DeFranco D. Mechanism of action of Hic-5/androgen receptor activator 55, a LIM domain-containing nuclear receptor coactivator. Mol Endocrinol. 2006;20:56-64 pubmed
| |
Barish G, Downes M, Alaynick W, Yu R, Ocampo C, Bookout A, et al. A Nuclear Receptor Atlas: macrophage activation. Mol Endocrinol. 2005;19:2466-77 pubmed
| |
Carrigan P, Riggs D, Chinkers M, Smith D. Functional comparison of human and Drosophila Hop reveals novel role in steroid receptor maturation. J Biol Chem. 2005;280:8906-11 pubmed
| |
Brkljacic J, Milutinović D, Dundjerski J, Matić G. Mercury stimulates rat liver glucocorticoid receptor association with Hsp90 and Hsp70. J Biochem Mol Toxicol. 2004;18:257-60 pubmed
| |
Cho S, Kagan B, Blackford J, Szapary D, Simons S. Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and Ubc9. Mol Endocrinol. 2005;19:290-311 pubmed
| |
Harrell J, Murphy P, Morishima Y, Chen H, Mansfield J, Galigniana M, et al. Evidence for glucocorticoid receptor transport on microtubules by dynein. J Biol Chem. 2004;279:54647-54 pubmed
| |
Wei Q, Lu X, Liu L, Schafer G, Shieh K, Burke S, et al. Glucocorticoid receptor overexpression in forebrain: a mouse model of increased emotional lability. Proc Natl Acad Sci U S A. 2004;101:11851-6 pubmed
| |
Billecke S, Draganov D, Morishima Y, Murphy P, Dunbar A, Pratt W, et al. The role of hsp90 in heme-dependent activation of apo-neuronal nitric-oxide synthase. J Biol Chem. 2004;279:30252-8 pubmed
| |
Hoetzenecker W, Meingassner J, Ecker R, Stingl G, Stuetz A, Elbe Bürger A. Corticosteroids but not pimecrolimus affect viability, maturation and immune function of murine epidermal Langerhans cells. J Invest Dermatol. 2004;122:673-84 pubmed
| |
Nelson G, Prapapanich V, Carrigan P, Roberts P, Riggs D, Smith D. The heat shock protein 70 cochaperone hip enhances functional maturation of glucocorticoid receptor. Mol Endocrinol. 2004;18:1620-30 pubmed
| |
Wang Q, Blackford J, Song L, Huang Y, Cho S, Simons S. Equilibrium interactions of corepressors and coactivators with agonist and antagonist complexes of glucocorticoid receptors. Mol Endocrinol. 2004;18:1376-95 pubmed
| |
Kutzler M, Ruane E, Coksaygan T, Vincent S, Nathanielsz P. Effects of three courses of maternally administered dexamethasone at 0.7, 0.75, and 0.8 of gestation on prenatal and postnatal growth in sheep. Pediatrics. 2004;113:313-9 pubmed
| |
Viegas L, Vicent G, Barañao J, Beato M, Pecci A. Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4. J Biol Chem. 2004;279:9831-9 pubmed
| |
Morishima Y, Kanelakis K, Murphy P, Lowe E, Jenkins G, Osawa Y, et al. The hsp90 cochaperone p23 is the limiting component of the multiprotein hsp90/hsp70-based chaperone system in vivo where it acts to stabilize the client protein: hsp90 complex. J Biol Chem. 2003;278:48754-63 pubmed
| |
Hoijman E, Rocha Viegas L, Keller Sarmiento M, Rosenstein R, Pecci A. Involvement of Bax protein in the prevention of glucocorticoid-induced thymocytes apoptosis by melatonin. Endocrinology. 2004;145:418-25 pubmed
| |
Kasai M, Guerrero Santoro J, Friedman R, Leman E, Getzenberg R, DeFranco D. The Group 3 LIM domain protein paxillin potentiates androgen receptor transactivation in prostate cancer cell lines. Cancer Res. 2003;63:4927-35 pubmed
| |
Tjandra K, Le T, Swain M. Glucocorticoid receptors are downregulated in hepatic T lymphocytes in rats with experimental cholangitis. Gut. 2003;52:1363-70 pubmed
| |
Walther R, Lamprecht C, Ridsdale A, Groulx I, Lee S, Lefebvre Y, et al. Nuclear export of the glucocorticoid receptor is accelerated by cell fusion-dependent release of calreticulin. J Biol Chem. 2003;278:37858-64 pubmed
| |
Murphy P, Morishima Y, Chen H, Galigniana M, Mansfield J, Simons S, et al. Visualization and mechanism of assembly of a glucocorticoid receptor.Hsp70 complex that is primed for subsequent Hsp90-dependent opening of the steroid binding cleft. J Biol Chem. 2003;278:34764-73 pubmed
| |
Lambert J, Nordeen S. CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins. Mol Endocrinol. 2003;17:1085-94 pubmed
| |
Patel A, Bulloch K. Type II glucocorticoid receptor immunoreactivity in the mossy cells of the rat and the mouse hippocampus. Hippocampus. 2003;13:59-66 pubmed
| |
Riggs D, Roberts P, Chirillo S, Cheung Flynn J, Prapapanich V, Ratajczak T, et al. The Hsp90-binding peptidylprolyl isomerase FKBP52 potentiates glucocorticoid signaling in vivo. EMBO J. 2003;22:1158-67 pubmed
| |
Li Q, Su A, Chen J, Lefebvre Y, Haché R. Attenuation of glucocorticoid signaling through targeted degradation of p300 via the 26S proteasome pathway. Mol Endocrinol. 2002;16:2819-27 pubmed
| |
Brewer J, Kanagawa O, Sleckman B, Muglia L. Thymocyte apoptosis induced by T cell activation is mediated by glucocorticoids in vivo. J Immunol. 2002;169:1837-43 pubmed
| |
Kanelakis K, Shewach D, Pratt W. Nucleotide binding states of hsp70 and hsp90 during sequential steps in the process of glucocorticoid receptor.hsp90 heterocomplex assembly. J Biol Chem. 2002;277:33698-703 pubmed
| |
Harrell J, Kurek I, Breiman A, Radanyi C, Renoir J, Pratt W, et al. All of the protein interactions that link steroid receptor.hsp90.immunophilin heterocomplexes to cytoplasmic dynein are common to plant and animal cells. Biochemistry. 2002;41:5581-7 pubmed
| |
Piwien Pilipuk G, Kanelakis K, Ghini A, Lantos C, Litwack G, Burton G, et al. Modification of an essential amino group in the mineralocorticoid receptor evidences a differential conformational change of the receptor protein upon binding of antagonists, natural agonists and the synthetic agonist 11,19-oxidoprogesterone. Biochim Biophys Acta. 2002;1589:31-48 pubmed
| |
Murphy E, Spencer R, Sipe K, Herman J. Decrements in nuclear glucocorticoid receptor (GR) protein levels and DNA binding in aged rat hippocampus. Endocrinology. 2002;143:1362-70 pubmed
| |
Davies T, Ning Y, SANCHEZ E. A new first step in activation of steroid receptors: hormone-induced switching of FKBP51 and FKBP52 immunophilins. J Biol Chem. 2002;277:4597-600 pubmed
| |
Proulx K, Clavel S, Nault G, Richard D, Walker C. High neonatal leptin exposure enhances brain GR expression and feedback efficacy on the adrenocortical axis of developing rats. Endocrinology. 2001;142:4607-16 pubmed
| |
Mukhopadhyay S, Wyszomierski S, Gronostajski R, Rosen J. Differential interactions of specific nuclear factor I isoforms with the glucocorticoid receptor and STAT5 in the cooperative regulation of WAP gene transcription. Mol Cell Biol. 2001;21:6859-69 pubmed
| |
Soubeyrand S, Torrance H, Giffin W, Gong W, Schild Poulter C, Hache R. Activation and autoregulation of DNA-PK from structured single-stranded DNA and coding end hairpins. Proc Natl Acad Sci U S A. 2001;98:9605-10 pubmed
| |
Biola A, Lefebvre P, Perrin Wolff M, Sturm M, Bertoglio J, Pallardy M. Interleukin-2 inhibits glucocorticoid receptor transcriptional activity through a mechanism involving STAT5 (signal transducer and activator of transcription 5) but not AP-1. Mol Endocrinol. 2001;15:1062-76 pubmed
| |
Murphy P, Kanelakis K, Galigniana M, Morishima Y, Pratt W. Stoichiometry, abundance, and functional significance of the hsp90/hsp70-based multiprotein chaperone machinery in reticulocyte lysate. J Biol Chem. 2001;276:30092-8 pubmed
| |
Belikov S, Gelius B, Wrange O. Hormone-induced nucleosome positioning in the MMTV promoter is reversible. EMBO J. 2001;20:2802-11 pubmed
| |
Mullick J, Anandatheerthavarada H, Amuthan G, Bhagwat S, Biswas G, Camasamudram V, et al. Physical interaction and functional synergy between glucocorticoid receptor and Ets2 proteins for transcription activation of the rat cytochrome P-450c27 promoter. J Biol Chem. 2001;276:18007-17 pubmed
| |
Morin B, Woodcock G, Nichols L, Holland L. Heterodimerization between the glucocorticoid receptor and the unrelated DNA-binding protein, Xenopus glucocorticoid receptor accessory factor. Mol Endocrinol. 2001;15:458-66 pubmed
| |
Wyszomierski S, Rosen J. Cooperative effects of STAT5 (signal transducer and activator of transcription 5) and C/EBPbeta (CCAAT/enhancer-binding protein-beta) on beta-casein gene transcription are mediated by the glucocorticoid receptor. Mol Endocrinol. 2001;15:228-40 pubmed
| |
Savory J, Préfontaine G, Lamprecht C, Liao M, Walther R, Lefebvre Y, et al. Glucocorticoid receptor homodimers and glucocorticoid-mineralocorticoid receptor heterodimers form in the cytoplasm through alternative dimerization interfaces. Mol Cell Biol. 2001;21:781-93 pubmed
| |
Kanelakis K, Murphy P, Galigniana M, Morishima Y, Takayama S, Reed J, et al. hsp70 interacting protein Hip does not affect glucocorticoid receptor folding by the hsp90-based chaperone machinery except to oppose the effect of BAG-1. Biochemistry. 2000;39:14314-21 pubmed
| |
Rajapandi T, Greene L, Eisenberg E. The molecular chaperones Hsp90 and Hsc70 are both necessary and sufficient to activate hormone binding by glucocorticoid receptor. J Biol Chem. 2000;275:22597-604 pubmed
| |
Johnson J, Craig E. A role for the Hsp40 Ydj1 in repression of basal steroid receptor activity in yeast. Mol Cell Biol. 2000;20:3027-36 pubmed
| |
Morishima Y, Kanelakis K, Silverstein A, Dittmar K, Estrada L, Pratt W. The Hsp organizer protein hop enhances the rate of but is not essential for glucocorticoid receptor folding by the multiprotein Hsp90-based chaperone system. J Biol Chem. 2000;275:6894-900 pubmed
| |
Giannoukos G, Silverstein A, Pratt W, Simons S. The seven amino acids (547-553) of rat glucocorticoid receptor required for steroid and hsp90 binding contain a functionally independent LXXLL motif that is critical for steroid binding. J Biol Chem. 1999;274:36527-36 pubmed
| |
Kanelakis K, Morishima Y, Dittmar K, Galigniana M, Takayama S, Reed J, et al. Differential effects of the hsp70-binding protein BAG-1 on glucocorticoid receptor folding by the hsp90-based chaperone machinery. J Biol Chem. 1999;274:34134-40 pubmed
| |
Sacedon R, Vicente A, Varas A, Jimenez E, Munoz J, Zapata A. Early maturation of T-cell progenitors in the absence of glucocorticoids. Blood. 1999;94:2819-26 pubmed
| |
Herman J, Watson S, Spencer R. Defense of adrenocorticosteroid receptor expression in rat hippocampus: effects of stress and strain. Endocrinology. 1999;140:3981-91 pubmed
| |
Sacedon R, Vicente A, Varas A, Jimenez E, Munoz J, Zapata A. Glucocorticoid-mediated regulation of thymic dendritic cell function. Int Immunol. 1999;11:1217-24 pubmed
| |
Towns R, Menon K, Brabec R, Silverstein A, Cohen J, Bowen J, et al. Glucocorticoids stimulate the accumulation of lipids in the rat corpus luteum. Biol Reprod. 1999;61:416-21 pubmed
| |
Galigniana M, Housley P, DeFranco D, Pratt W. Inhibition of glucocorticoid receptor nucleocytoplasmic shuttling by okadaic acid requires intact cytoskeleton. J Biol Chem. 1999;274:16222-7 pubmed
| |
Nogami H, Inoue K, Moriya H, Ishida A, Kobayashi S, Hisano S, et al. Regulation of growth hormone-releasing hormone receptor messenger ribonucleic acid expression by glucocorticoids in MtT-S cells and in the pituitary gland of fetal rats. Endocrinology. 1999;140:2763-70 pubmed
| |
Reynolds P, Ruan Y, Smith D, Scammell J. Glucocorticoid resistance in the squirrel monkey is associated with overexpression of the immunophilin FKBP51. J Clin Endocrinol Metab. 1999;84:663-9 pubmed
| |
Wyszomierski S, Yeh J, Rosen J. Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation. Mol Endocrinol. 1999;13:330-43 pubmed
| |
Jun S, Chen Z, Pace M, Shaul P. Glucocorticoids downregulate cyclooxygenase-1 gene expression and prostacyclin synthesis in fetal pulmonary artery endothelium. Circ Res. 1999;84:193-200 pubmed
| |
Savory J, Hsu B, Laquian I, Giffin W, Reich T, Hache R, et al. Discrimination between NL1- and NL2-mediated nuclear localization of the glucocorticoid receptor. Mol Cell Biol. 1999;19:1025-37 pubmed
| |
Chandran U, Warren B, Baumann C, Hager G, DeFranco D. The glucocorticoid receptor is tethered to DNA-bound Oct-1 at the mouse gonadotropin-releasing hormone distal negative glucocorticoid response element. J Biol Chem. 1999;274:2372-8 pubmed
| |
Bender A, Silverstein A, Demady D, Kanelakis K, Noguchi S, Pratt W, et al. Neuronal nitric-oxide synthase is regulated by the Hsp90-based chaperone system in vivo. J Biol Chem. 1999;274:1472-8 pubmed
| |
Hache R, Tse R, Reich T, Savory J, Lefebvre Y. Nucleocytoplasmic trafficking of steroid-free glucocorticoid receptor. J Biol Chem. 1999;274:1432-9 pubmed
| |
Herman J, Spencer R. Regulation of hippocampal glucocorticoid receptor gene transcription and protein expression in vivo. J Neurosci. 1998;18:7462-73 pubmed
| |
Tang Y, Getzenberg R, Vietmeier B, Stallcup M, Eggert M, Renkawitz R, et al. The DNA-binding and tau2 transactivation domains of the rat glucocorticoid receptor constitute a nuclear matrix-targeting signal. Mol Endocrinol. 1998;12:1420-31 pubmed
| |
Caamaño C, Morano M, Dalman F, Pratt W, Akil H. A conserved proline in the hsp90 binding region of the glucocorticoid receptor is required for hsp90 heterocomplex stabilization and receptor signaling. J Biol Chem. 1998;273:20473-80 pubmed
| |
Dittmar K, Banach M, Galigniana M, Pratt W. The role of DnaJ-like proteins in glucocorticoid receptor.hsp90 heterocomplex assembly by the reconstituted hsp90.p60.hsp70 foldosome complex. J Biol Chem. 1998;273:7358-66 pubmed
| |
Barent R, Nair S, Carr D, Ruan Y, Rimerman R, Fulton J, et al. Analysis of FKBP51/FKBP52 chimeras and mutants for Hsp90 binding and association with progesterone receptor complexes. Mol Endocrinol. 1998;12:342-54 pubmed
| |
Dittmar K, Demady D, Stancato L, Krishna P, Pratt W. Folding of the glucocorticoid receptor by the heat shock protein (hsp) 90-based chaperone machinery. The role of p23 is to stabilize receptor.hsp90 heterocomplexes formed by hsp90.p60.hsp70. J Biol Chem. 1997;272:21213-20 pubmed
| |
Silverstein A, Galigniana M, Chen M, Owens Grillo J, Chinkers M, Pratt W. Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin. J Biol Chem. 1997;272:16224-30 pubmed
| |
Dittmar K, Pratt W. Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation. J Biol Chem. 1997;272:13047-54 pubmed
| |
Sackey F, Hache R, Reich T, Kwast Welfeld J, Lefebvre Y. Determinants of subcellular distribution of the glucocorticoid receptor. Mol Endocrinol. 1996;10:1191-205 pubmed
| |
Hong H, Kohli K, Garabedian M, Stallcup M. GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors. Mol Cell Biol. 1997;17:2735-44 pubmed
| |
Chang H, Nathan D, Lindquist S. In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Mol Cell Biol. 1997;17:318-25 pubmed
| |
Chen M, Silverstein A, Pratt W, Chinkers M. The tetratricopeptide repeat domain of protein phosphatase 5 mediates binding to glucocorticoid receptor heterocomplexes and acts as a dominant negative mutant. J Biol Chem. 1996;271:32315-20 pubmed
| |
Dittmar K, Hutchison K, Owens Grillo J, Pratt W. Reconstitution of the steroid receptor.hsp90 heterocomplex assembly system of rabbit reticulocyte lysate. J Biol Chem. 1996;271:12833-9 pubmed
| |
Curtis L, Rarey K. Effect of stress on cochlear glucocorticoid protein. II. Restraint. Hear Res. 1995;92:120-5 pubmed
| |
Huss J, Wang S, Astrom A, McQuiddy P, Kasper C. Dexamethasone responsiveness of a major glucocorticoid-inducible CYP3A gene is mediated by elements unrelated to a glucocorticoid receptor binding motif. Proc Natl Acad Sci U S A. 1996;93:4666-70 pubmed
| |
Stancato L, Silverstein A, Gitler C, Groner B, Pratt W. Use of the thiol-specific derivatizing agent N-iodoacetyl-3-[125I]iodotyrosine to demonstrate conformational differences between the unbound and hsp90-bound glucocorticoid receptor hormone binding domain. J Biol Chem. 1996;271:8831-6 pubmed
| |
Chang T, Scher B, Waxman S, Scher W. Inhibition of mouse GATA-1 function by the glucocorticoid receptor: possible mechanism of steroid inhibition of erythroleukemia cell differentiation. Mol Endocrinol. 1993;7:528-42 pubmed
| |
Stancato L, Chow Y, Hutchison K, Perdew G, Jove R, Pratt W. Raf exists in a native heterocomplex with hsp90 and p50 that can be reconstituted in a cell-free system. J Biol Chem. 1993;268:21711-6 pubmed
| |
Opoku J, Simons S. Absence of intramolecular disulfides in the structure and function of native rat glucocorticoid receptors. J Biol Chem. 1994;269:503-10 pubmed
| |
Czar M, Owens Grillo J, Dittmar K, Hutchison K, Zacharek A, Leach K, et al. Characterization of the protein-protein interactions determining the heat shock protein (hsp90.hsp70.hsp56) heterocomplex. J Biol Chem. 1994;269:11155-61 pubmed
| |
Hutchison K, Dittmar K, Czar M, Pratt W. Proof that hsp70 is required for assembly of the glucocorticoid receptor into a heterocomplex with hsp90. J Biol Chem. 1994;269:5043-9 pubmed
| |
Smets L, van Den Berg J, Acton D, Top B, Van Rooij H, Verwijs Janssen M. BCL-2 expression and mitochondrial activity in leukemic cells with different sensitivity to glucocorticoid-induced apoptosis. Blood. 1994;84:1613-9 pubmed
| |
Kupfer S, Wilson E, French F. Androgen and glucocorticoid receptors interact with insulin degrading enzyme. J Biol Chem. 1994;269:20622-8 pubmed
| |
Garlatti M, Daheshia M, Slater E, Bouguet J, Hanoune J, Beato M, et al. A functional glucocorticoid-responsive unit composed of two overlapping inactive receptor-binding sites: evidence for formation of a receptor tetramer. Mol Cell Biol. 1994;14:8007-17 pubmed
| |
Hutchison K, Dittmar K, Pratt W. All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a "foldosome". J Biol Chem. 1994;269:27894-9 pubmed
| |
van den Berg J, Smets L, Hutchison K, Van Rooij H, van den Elshout M. High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone. J Steroid Biochem Mol Biol. 1994;51:33-40 pubmed
| |
Modarress K, Cavanaugh A, Chakraborti P, Simons S. Metal oxyanion stabilization of the rat glucocorticoid receptor is independent of thiols. J Biol Chem. 1994;269:25621-8 pubmed
| |
Chang H, Lindquist S. Conservation of Hsp90 macromolecular complexes in Saccharomyces cerevisiae. J Biol Chem. 1994;269:24983-8 pubmed
| |
Rarey K, Gerhardt K, Curtis L, ten Cate W. Effect of stress on cochlear glucocorticoid protein: acoustic stress. Hear Res. 1995;82:135-8 pubmed
| |
Owens Grillo J, Hoffmann K, Hutchison K, Yem A, Deibel M, Handschumacher R, et al. The cyclosporin A-binding immunophilin CyP-40 and the FK506-binding immunophilin hsp56 bind to a common site on hsp90 and exist in independent cytosolic heterocomplexes with the untransformed glucocorticoid receptor. J Biol Chem. 1995;270:20479-84 pubmed
| |
Hutchison K, Stancato L, Owens Grillo J, Johnson J, Krishna P, Toft D, et al. The 23-kDa acidic protein in reticulocyte lysate is the weakly bound component of the hsp foldosome that is required for assembly of the glucocorticoid receptor into a functional heterocomplex with hsp90. J Biol Chem. 1995;270:18841-7 pubmed
| |
Renoir J, Mercier Bodard C, Hoffmann K, Le Bihan S, Ning Y, Sanchez E, et al. Cyclosporin A potentiates the dexamethasone-induced mouse mammary tumor virus-chloramphenicol acetyltransferase activity in LMCAT cells: a possible role for different heat shock protein-binding immunophilins in glucocorticosteroid receptor-mediated g. Proc Natl Acad Sci U S A. 1995;92:4977-81 pubmed
| |
Tanaka H, Makino Y, Dahlman Wright K, Gustafsson J, Okamoto K, Makino I, et al. Zinc ions antagonize the inhibitory effect of aurothiomalate on glucocorticoid receptor function at physiological concentrations. Mol Pharmacol. 1995;48:938-45 pubmed
| |
Gametchu B, Harrison R. Characterization of a monoclonal antibody to the rat liver glucocorticoid receptor. Endocrinology. 1984;114:274-9 pubmed
| |
Rehmus E, Howard K, Janiga K, Distelhorst C. Immunochemical comparison of mutant glucocorticoid receptors and wild type receptor fragments produced by neutrophil elastase and chymotrypsin. J Steroid Biochem. 1987;28:167-77 pubmed
| |
Hendry W, Danzo B, Harrison R. Analysis of the disruptive action of an epididymal protease and the stabilizing influence of molybdate on nondenatured and denatured glucocorticoid receptor. Endocrinology. 1987;120:629-39 pubmed
| |
Bresnick E, Sanchez E, Pratt W. Relationship between glucocorticoid receptor steroid-binding capacity and association of the Mr 90,000 heat shock protein with the unliganded receptor. J Steroid Biochem. 1988;30:267-9 pubmed
| |
LaFond R, Kennedy S, Harrison R, Villee C. Immunocytochemical localization of glucocorticoid receptors in cells, cytoplasts, and nucleoplasts. Exp Cell Res. 1988;175:52-62 pubmed
| |
Howard K, Distelhorst C. Evidence for intracellular association of the glucocorticoid receptor with the 90-kDa heat shock protein. J Biol Chem. 1988;263:3474-81 pubmed
| |
Orti E, Mendel D, Munck A. Phosphorylation of glucocorticoid receptor-associated and free forms of the approximately 90-kDa heat shock protein before and after receptor activation. J Biol Chem. 1989;264:231-7 pubmed
| |
Distelhorst C, Howard K. Kinetic pulse-chase labeling study of the glucocorticoid receptor in mouse lymphoma cells. Effect of glucocorticoid and antiglucocorticoid hormones on intracellular receptor half-life. J Biol Chem. 1989;264:13080-5 pubmed
| |
Raaka B, Finnerty M, Samuels H. The glucocorticoid antagonist 17 alpha-methyltestosterone binds to the 10 S glucocorticoid receptor and blocks agonist-mediated dissociation of the 10 S oligomer to the 4 S deoxyribonucleic acid-binding subunit. Mol Endocrinol. 1989;3:332-41 pubmed
| |
Unger A, Uppaluri R, Ahern S, Colby J, Tymoczko J. Isolation of ribonucleic acid from the unactivated rat liver glucocorticoid receptor. Mol Endocrinol. 1988;2:952-8 pubmed
| |
Bresnick E, Dalman F, Pratt W. Direct stoichiometric evidence that the untransformed Mr 300,000, 9S, glucocorticoid receptor is a core unit derived from a larger heteromeric complex. Biochemistry. 1990;29:520-7 pubmed
| |
Schlatter L, Howard K, Parker M, Distelhorst C. Comparison of the 90-kilodalton heat shock protein interaction with in vitro translated glucocorticoid and estrogen receptors. Mol Endocrinol. 1992;6:132-40 pubmed
| |
Yoshinaga S, Peterson C, Herskowitz I, Yamamoto K. Roles of SWI1, SWI2, and SWI3 proteins for transcriptional enhancement by steroid receptors. Science. 1992;258:1598-604 pubmed
| |
Hutchison K, Brott B, De Leon J, Perdew G, Jove R, Pratt W. Reconstitution of the multiprotein complex of pp60src, hsp90, and p50 in a cell-free system. J Biol Chem. 1992;267:2902-8 pubmed
|
图像
图像 1 :
![赛默飞世尔 MA1-510 图像 1](http://www.labome.com/product/Invitrogen/2228454_0.jpg)
Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) (Product# MA1-510 ) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor (Product# MA1-510 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
![赛默飞世尔 MA1-510 图像 2](http://www.labome.com/product/Invitrogen/2228454_1.jpg)
Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) (Product# MA1-510 ) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor (Product# MA1-510 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 3 :
![赛默飞世尔 MA1-510 图像 3](http://www.labome.com/product/Invitrogen/2228454_2.jpg)
产品信息
产品类型 :
抗体
产品名称 :
糖皮质激素受体单克隆抗体(BuGR2)
目录# :
MA1-510
规格 :
100微克
价格 :
美国446.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Guinea Pig, Human, Mouse, Ovine, Rabbit, Rat, Yeast
应用 :
Flow Cytometry: 1-2 µg/test, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 5 µg/mL, Immunohistochemistry (PFA fixed): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 5 µg/mL
物种 :
Guinea Pig, Human, Mouse, Ovine, Rabbit, Rat, Yeast
克隆 :
BuGR2
抗体亚型 :
IgG2a
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
免疫原 :
Partially purified rat GR.
格式 :
冻干
应用w/稀释 :
Flow Cytometry: 1-2 µg/test, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 5 µg/mL, Immunohistochemistry (PFA fixed): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 5 µg/mL
别名 :
cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/72.jpg)
赛默飞世尔