产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化CREB/ATF1(Ser133, Ser63)单克隆抗体(10E9)
目录 :
MA1-114
规格 :
100微克
价格 :
美国626.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
10E9
反应物种 :
人类, 小鼠, 犬
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
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文章摘录数: 1
图像
图像 1 :
Immunofluorescent analysis of Phospho-CREB pSer133 (green) in HeLa cells either left untreated (left panel) or treated with 1uM Camptothecin (right panel) for 20 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-CREB pSer133 monoclonal antibody (Product # MA1-114) at a concentration of 4ug/ml for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight Instrument at 20X magnification.
产品信息
产品类型 :
抗体
产品名称 :
磷酸化CREB/ATF1(Ser133, Ser63)单克隆抗体(10E9)
目录# :
MA1-114
规格 :
100微克
价格 :
美国626.00
克隆性 :
单克隆
纯度 :
尺寸排阻色谱法
宿主 :
小鼠
反应物种 :
狗, 人类, 小鼠
应用 :
ChIP Assay: 5 µL/10^6 cells, ELISA: 0.05 µg/mL, Flow Cytometry: 1 µg/test, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:500
物种 :
狗, 人类, 小鼠
克隆 :
10E9
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory.
免疫原 :
A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB
格式 :
液体
应用w/稀释 :
ChIP Assay: 5 µL/10^6 cells, ELISA: 0.05 µg/mL, Flow Cytometry: 1 µg/test, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:500
别名 :
2310001E10Rik; 3526402H21Rik; Activating transcription factor 1; active transcription factor CREB; ATF1; AV083133; cAMP responsive element binding protein 1; cAMP-dependent transcription factor ATF-1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; Creb; CREB1; Creb-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-dependent transcription factor ATF-1; Cyclic AMP-responsive element-binding protein 1; EWS-ATF1; FUS/ATF-1; Protein TREB36; Ser133; TCR-ATF1; transactivator protein; TREB36
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公司信息
赛默飞世尔