FKBP4多克隆抗体
赛默飞世尔
目录: PA3-020
domestic rabbit 多克隆
反应物种: 人类, 小鼠, domestic rabbit
应用: 免疫印迹, 免疫细胞化学

Western blot detection of Hsp56 on human cell line S49 cytosol using PA3-020.

Western blot analysis of Heat Shock Protein 56 (HSP56) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunofluorescent analysis of Heat Shock protein 56 (HSP56, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
规格: 100微克
价格: 美国420.00
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FKBP4多克隆抗体
赛默飞世尔
目录: PA5-19057
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
FKBP4重组兔单克隆抗体(JG96-30)
赛默飞世尔
目录: MA5-34801
domestic rabbit 重组 (JG96-30)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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FKBP4重组兔单克隆抗体(ARC0977)
赛默飞世尔
目录: MA5-35491
domestic rabbit 重组 (ARC0977)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国446.00
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磷酸化FKBP4(Tyr220)多克隆抗体
赛默飞世尔
目录: PA5-105340
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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FKBP4单克隆抗体(Hi52C), APC
赛默飞世尔
目录: MA5-45163
小鼠 单克隆 (Hi52C)
反应物种: 中国人仓鼠, 人类, 小鼠, 大鼠,
共轭标签: APC
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
规格: 100微克
价格: 美国426.00
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小鼠 单克隆 (Hi52C)
反应物种: 中国人仓鼠, 人类, 小鼠, 大鼠,
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
规格: 100微克
价格: 美国416.00
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FKBP4单克隆抗体(Hi52C), PerCP
赛默飞世尔
目录: MA5-45165
小鼠 单克隆 (Hi52C)
反应物种: 中国人仓鼠, 人类, 小鼠, 大鼠,
共轭标签: PerCP
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
规格: 100微克
价格: 美国426.00
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FKBP4多克隆抗体
赛默飞世尔
目录: PA5-119118
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 200微升
价格: 美国416.00
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FKBP4多克隆抗体
赛默飞世尔
目录: PA5-120934
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
规格: 100微升
价格: 美国440.00
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FKBP4多克隆抗体
赛默飞世尔
目录: PA5-121985
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 20微升
价格: 美国169.00
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