Presenilin 1 Monoclonal Antibody (APS 18)
赛默飞世尔
目录: MA1-752
小鼠 单克隆 (APS 18)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
文章摘录数: 6

Immunofluorescent analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) (Product# MA1-752) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 (Product# MA1-752) at a dilution of 1:20-1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-752) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-752) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
规格: 200微克
价格: 美国430.00
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Presenilin 1 Monoclonal Antibody (APS 11)
赛默飞世尔
目录: MA1-751
小鼠 单克隆 (APS 11)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 1


Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
规格: 200微克
价格: 美国450.00
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
文章摘录数: 1
规格: 100微升
价格: 美国436.00
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Presenilin 1 Polyclonal Antibody
赛默飞世尔
目录: PA5-96088
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 1
规格: 100微升
价格: 美国446.00
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domestic rabbit 重组 (ARC0440)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
规格: 100微升
价格: 美国446.00
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Phospho-Presenilin 1 (Ser353) Polyclonal Antibody
赛默飞世尔
目录: PA5-118711
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国416.00
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Presenilin 1 Polyclonal Antibody
赛默飞世尔
目录: PA5-119872
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国440.00
至产商