产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
整合素αⅤ亚单位(CD51, 纤维连接蛋白受体甲亚单位)
目录 :
P3G8
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
P3G8
反应物种 :
人类
应用 :
酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
文章摘录数: 11
参考文献
Kamata T, Handa M, Takakuwa S, Sato Y, Kawai Y, Ikeda Y, et al. Epitope mapping for monoclonal antibody reveals the activation mechanism for ?V?3 integrin. PLoS ONE. 2013;8:e66096 pubmed 出版商
Kirkland S, Ying H. Alpha2beta1 integrin regulates lineage commitment in multipotent human colorectal cancer cells. J Biol Chem. 2008;283:27612-9 pubmed 出版商
Zhang Y, Lu H, Dazin P, Kapila Y. Functional differences between integrin alpha4 and integrins alpha5/alphav in modulating the motility of human oral squamous carcinoma cells in response to the V region and heparin-binding domain of fibronectin. Exp Cell Res. 2004;295:48-58 pubmed
Ellis P, Metcalfe J, Hyvonen M, Kemp P. Adhesion of endothelial cells to NOV is mediated by the integrins alphavbeta3 and alpha5beta1. J Vasc Res. 2003;40:234-43 pubmed
Kim J, Jeong H, Nam J, Lee B, Choi J, Park R, et al. Identification of motifs in the fasciclin domains of the transforming growth factor-beta-induced matrix protein betaig-h3 that interact with the alphavbeta5 integrin. J Biol Chem. 2002;277:46159-65 pubmed
Vihinen P, Nikkola J, Vlaykova T, Hahka Kemppinen M, Talve L, Heino J, et al. Prognostic value of beta1 integrin expression in metastatic melanoma. Melanoma Res. 2000;10:243-51 pubmed
Skubitz A, Grossman M, McCarthy J, Wayner E, Cameron J. The decreased adhesion of Y79 retinoblastoma cells to extracellular matrix proteins is due to a deficit of integrin receptors. Invest Ophthalmol Vis Sci. 1994;35:2820-33 pubmed
Weinacker A, Chen A, Agrez M, Cone R, Nishimura S, Wayner E, et al. Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor. J Biol Chem. 1994;269:6940-8 pubmed
Maldonado B, Furcht L. Involvement of integrins with adhesion-promoting, heparin-binding peptides of type IV collagen in cultured human corneal epithelial cells. Invest Ophthalmol Vis Sci. 1995;36:364-72 pubmed
Levesque J, Leavesley D, Niutta S, Vadas M, Simmons P. Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins. J Exp Med. 1995;181:1805-15 pubmed
Wayner E, Orlando R, Cheresh D. Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol. 1991;113:919-29 pubmed
产品信息
内部号码 :
7926
名称 :
P3G8
Depositor Name :
Wayner, E.A.
Depositor Institution :
Fred Hutchison Cancer Research Center
Date Deposited :
10/11/11
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
整合素αⅤ亚单位(CD51, 纤维连接蛋白受体甲亚单位)
抗原物种 :
人类
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
人类
Species Tested (legacy) :
人类
Initial Publication Pubmed ID :
1709170
Depositor Notes (Special Instructions) :
Immunostaining: Fixation, frozen (acetone), paraformaldehyde; Paraffin, yes – formalin fixed; Special protocols, Antigen Retrieval with 10mM sodium citrate pH 6.0. Doesn't appear to work for Immunoblotting.
Collections :
CD antigens,Cell signaling,Human
搜索关键词 :
Wayner, E.A., integrin alpha V subunit (CD51, fibronectin receptor alpha subunit), Human, MIgG1, Human, ITGAV, MSK8, VNRA, CD51, VTNR, AB_2619589, monoclonal, Human/Cell signaling/Cluster Determinant antigens, Immunohistochemistry/FFPE/Immunoprecipitation/ELISA/FACS
抗原分子量 :
Predicted: 111 to 116 kDa
基因 :
ITGAV
Alternate Gene Name(s) :
MSK8, VNRA, CD51, VTNR
UniProt数据库编号 :
P06756
抗体登记处号码 :
AB_2619589
Synonyms (Alternate Clone Names) :
CD51
免疫原 :
Small lung carcinoma cells (UCLA P3).
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
NS-1/狐狸-NY
表位定位 :
Yes
Epitope Location or Sequence :
The epitope is located in the extracellular region; aa 528 of human alpha-v integrin (aa 558 of Uniprot P06756-1) is required for P3G8 binding.
表位定位Pubmed登录号 :
23840404
Recommended Applications :
酶联免疫吸附测定, 流式细胞仪, FFPE, 免疫组化, 免疫沉淀
Immunoblotting (legacy) :
no
免疫组化PubMed IDs :
10890378
免疫沉淀PubMed IDs :
8120056
流式细胞仪PubMed IDs :
8188477 15051489 7536795 23840404 12270930 7843907
PubMed IDs :
1709170 12902636 18664572
其他信息 :
P3G8 recognizes all isoforms.
DSHB增长培养基 :
RPMI
References (legacy) :
J. Biol. Chem. 269(9), 6940-6948.; J. Vasc. Res. 40, 234-243.; Exp. Cell Res. 295(1), 45-58.; J. Biol. Chem. 283, 27612-27619.
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。