产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
tropomyosin, striated muscle
目录 :
CH1
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
CH1
反应物种 :
jerboas, 人类, 小鼠, bantam, leopard danio, 非洲爪蛙
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
文章摘录数: 36
出版应用/物种/样本/稀释参考文献
  • 免疫组化-冰冻切片; jerboas; 1:10; 图 5a
Tran M, Tsutsumi R, Erberich J, Chen K, Flores M, Cooper K. Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death. elife. 2019;8: pubmed 出版商
  • 免疫组化-冰冻切片; leopard danio; 1:100; 图 s2
Sallin P, Jazwinska A. Acute stress is detrimental to heart regeneration in zebrafish. Open Biol. 2016;6: pubmed 出版商
  • 免疫组化; 非洲爪蛙; 1:25; 图 5
Tandon P, Wilczewski C, Williams C, Conlon F. The Lhx9-integrin pathway is essential for positioning of the proepicardial organ. Development. 2016;143:831-40 pubmed 出版商
  • 免疫细胞化学; 人类; 图 s5
Wang Y, Li Z, Zhang P, Poon E, Kong C, Boheler K, et al. Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes. Stem Cells. 2015;33:2973-84 pubmed 出版商
  • 免疫组化; 非洲爪蛙; 1:10; 图 1
Amin N, Gibbs D, Conlon F. Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development. Dev Dyn. 2014;243:948-56 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:100
Delgado Olguin P, Huang Y, Li X, Christodoulou D, Seidman C, Seidman J, et al. Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis. Nat Genet. 2012;44:343-7 pubmed 出版商
Crowell K, Laufenberg L, Lang C. Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function. Alcohol Clin Exp Res. 2019;43:2090-2099 pubmed 出版商
Kitakaze T, Sakamoto T, Kitano T, Inoue N, Sugihara F, Harada N, et al. The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy. Biochem Biophys Res Commun. 2016;478:1292-7 pubmed 出版商
Amarasinghe C, Hossain M, Jin J. Functional Basis of Three New Recessive Mutations of Slow Skeletal Muscle Troponin T Found in Non-Amish TNNT1 Nemaline Myopathies. Biochemistry. 2016;55:4560-7 pubmed 出版商
England J, Pang K, Parnall M, Haig M, Loughna S. Cardiac troponin T is necessary for normal development in the embryonic chick heart. J Anat. 2016;229:436-49 pubmed 出版商
Suzuki D, Fukumoto Y, Yoshimura M, Yamazaki Y, Kosaka J, Kuratani S, et al. Comparative morphology and development of extra-ocular muscles in the lamprey and gnathostomes reveal the ancestral state and developmental patterns of the vertebrate head. Zoological Lett. 2016;2:10 pubmed 出版商
Feng H, Chen X, Malek M, Jin J. Slow recovery of the impaired fatigue resistance in postunloading mouse soleus muscle corresponding to decreased mitochondrial function and a compensatory increase in type I slow fibers. Am J Physiol Cell Physiol. 2016;310:C27-40 pubmed 出版商
Wu Q, Zhang J, Koh W, Yu Q, Zhu X, Amsterdam A, et al. Talin1 is required for cardiac Z-disk stabilization and endothelial integrity in zebrafish. FASEB J. 2015;29:4989-5005 pubmed 出版商
Dube S, Yalamanchili S, Lachant J, Abbott L, Benz P, Mitschow C, et al. Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines. Int J Breast Cancer. 2015;2015:859427 pubmed 出版商
Pittner S, Monticelli F, Pfisterer A, Zissler A, Sänger A, Stoiber W, et al. Postmortem degradation of skeletal muscle proteins: a novel approach to determine the time since death. Int J Legal Med. 2016;130:421-31 pubmed 出版商
Amarasinghe C, Jin J. N-Terminal Hypervariable Region of Muscle Type Isoforms of Troponin T Differentially Modulates the Affinity of Tropomyosin-Binding Site 1. Biochemistry. 2015;54:3822-30 pubmed 出版商
Zajdel R, McLean M, Dube S, Dube D. Expression of tropomyosin in relation to myofibrillogenesis in axolotl hearts. Regen Med Res. 2013;1:8 pubmed 出版商
Wang X, Wang X, Yuan W, Chai R, Liu D. Egfl6 is involved in zebrafish notochord development. Fish Physiol Biochem. 2015;41:961-9 pubmed 出版商
Nan C, Dube S, Matoq A, Mikesell L, Abbott L, Alshiekh Nasany R, et al. Expression of sarcomeric tropomyosin in striated muscles in axolotl treated with shz-1, a small cardiogenic molecule. Cardiovasc Toxicol. 2015;15:29-40 pubmed 出版商
Marston S, Copeland O, Messer A, Macnamara E, Nowak K, Zampronio C, et al. Tropomyosin isoform expression and phosphorylation in the human heart in health and disease. J Muscle Res Cell Motil. 2013;34:189-97 pubmed 出版商
Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
Takeuchi J, Lou X, Alexander J, Sugizaki H, Delgado Olguin P, Holloway A, et al. Chromatin remodelling complex dosage modulates transcription factor function in heart development. Nat Commun. 2011;2:187 pubmed 出版商
Murphy R. Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1. J Appl Physiol (1985). 2011;110:820-5 pubmed 出版商
Biesiadecki B, Chong S, Nosek T, Jin J. Troponin T core structure and the regulatory NH2-terminal variable region. Biochemistry. 2007;46:1368-79 pubmed
Zhang Z, Biesiadecki B, Jin J. Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage. Biochemistry. 2006;45:11681-94 pubmed
Wang Y, Qian L, Yu Z, Jiang Q, Dong Y, Liu X, et al. Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility. FEBS Lett. 2005;579:4843-50 pubmed
Wang Y, Zhong T, Qian L, Dong Y, Jiang Q, Tan L, et al. Wortmannin induces zebrafish cardia bifida through a mechanism independent of phosphoinositide 3-kinase and myosin light chain kinase. Biochem Biophys Res Commun. 2005;331:303-8 pubmed
Denz C, Narshi A, Zajdel R, Dube D. Expression of a novel cardiac-specific tropomyosin isoform in humans. Biochem Biophys Res Commun. 2004;320:1291-7 pubmed
Kolker S, Tajchman U, Weeks D. Confocal imaging of early heart development in Xenopus laevis. Dev Biol. 2000;218:64-73 pubmed
Zajdel R, McLean M, Lemanski S, Muthuchamy M, Wieczorek D, Lemanski L, et al. Ectopic expression of tropomyosin promotes myofibrillogenesis in mutant axolotl hearts. Dev Dyn. 1998;213:412-20 pubmed
Begum S, Komiyama M, Toyota N, Obinata T, Maruyama K, Shimada Y. Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy. Cell Tissue Res. 1998;293:305-11 pubmed
L Ecuyer T, Noller J, Fulton A. Assembly of tropomyosin isoforms into the cytoskeleton of avian muscle cells. Pediatr Res. 1998;43:813-22 pubmed
Muthuchamy M, Grupp I, Grupp G, O Toole B, Kier A, Boivin G, et al. Molecular and physiological effects of overexpressing striated muscle beta-tropomyosin in the adult murine heart. J Biol Chem. 1995;270:30593-603 pubmed
Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
Lourim D, Lin J. Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro. J Cell Biol. 1989;109:495-504 pubmed
Maier A, Zak R. Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers. Histochemistry. 1990;93:423-8 pubmed
产品信息
内部号码 :
2988
名称 :
CH1
Depositor Name :
Lin, J.J.-C.
Depositor Institution :
University of Iowa
Date Deposited :
9/8/88
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
tropomyosin, striated muscle
抗原物种 :
鸡
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Axolotl,Broad species,Chicken,Fish,Human,Mouse,Rat,Xenopus
Species Tested (legacy) :
鸡, 大鼠, 小鼠, 蛙
Initial Publication Pubmed ID :
3899907
Collections :
Cardiac development,Cytoskeleton,Human,Muscular dystrophy,Skeletal muscle,Xenopus
搜索关键词 :
Lin, J.J.-C., tropomyosin, striated muscle, Chicken, MIgG1, chicken/rat/mouse/Xenopus/Human/Axolotl/fish/broad species, TPM1, , AB_2205770, monoclonal, Cardiac development/Muscular dystrophy/Skeletal muscle/Xenopus/Cytoskeletal elements/Human, Immunofluorescence/Western Blot/ELISA/FFPE/Function Blocking/Immunoprecipitation
抗原分子量 :
Apparent: 39 kDa (or smaller depending on the isoform)
基因 :
TPM1
UniProt数据库编号 :
P04268
抗体登记处号码 :
AB_2205770
免疫原 :
纯化鸡心脏肌肉原肌球蛋白
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
NS1
表位定位 :
Yes
Epitope Location or Sequence :
CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].
表位定位Pubmed登录号 :
24958154 22069507
Recommended Applications :
ELISA,FFPE,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
免疫荧光PubMed IDs :
15249230 9853962 26310270 25952972 27194630 27081572 25984327 24633745 2323957 9662653 10644411 16107252 15845393
免疫印迹PubMed IDs :
3899907 21088205 26171250 24958154 26041514 27553280 26447205 25984327 8530495 2668298 23712688 31411733
免疫沉淀PubMed IDs :
9621993
酶联免疫吸附测定PubMed IDs :
26024675 16981728 27429059 17260966
Function Blocking Pubmed IDs :
3899907
其他信息 :
CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.
DSHB增长培养基 :
RPMI
References (legacy) :
Hybridoma 4, 223-242.; Histochem. 93, 423-428.; J. Tiss. Cult. Meth. 14, 31-36.; Cell Tissue Res. 293, 305-311.; Dev. Biol. 218, 64-73.; FEBS Lett. 579, 4843-4850.; Biochem. Biophys. Res. Commun. 331, 303-308.
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构，为生命科学研究领域及诊断领域，提供高质量的抗体等产品。