产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
tropomyosin, striated muscle
目录 :
CH1
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
CH1
反应物种 :
jerboas, 人类, 小鼠, bantam, leopard danio, 非洲爪蛙
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片
文章摘录数: 36
出版应用/物种/样本/稀释 | 参考文献 |
---|---|
| |
| |
| |
| |
| |
| |
Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
| |
Biesiadecki B, Chong S, Nosek T, Jin J. Troponin T core structure and the regulatory NH2-terminal variable region. Biochemistry. 2007;46:1368-79 pubmed
| |
Zhang Z, Biesiadecki B, Jin J. Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage. Biochemistry. 2006;45:11681-94 pubmed
| |
Wang Y, Qian L, Yu Z, Jiang Q, Dong Y, Liu X, et al. Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility. FEBS Lett. 2005;579:4843-50 pubmed
| |
Wang Y, Zhong T, Qian L, Dong Y, Jiang Q, Tan L, et al. Wortmannin induces zebrafish cardia bifida through a mechanism independent of phosphoinositide 3-kinase and myosin light chain kinase. Biochem Biophys Res Commun. 2005;331:303-8 pubmed
| |
Denz C, Narshi A, Zajdel R, Dube D. Expression of a novel cardiac-specific tropomyosin isoform in humans. Biochem Biophys Res Commun. 2004;320:1291-7 pubmed
| |
Kolker S, Tajchman U, Weeks D. Confocal imaging of early heart development in Xenopus laevis. Dev Biol. 2000;218:64-73 pubmed
| |
Zajdel R, McLean M, Lemanski S, Muthuchamy M, Wieczorek D, Lemanski L, et al. Ectopic expression of tropomyosin promotes myofibrillogenesis in mutant axolotl hearts. Dev Dyn. 1998;213:412-20 pubmed
| |
Begum S, Komiyama M, Toyota N, Obinata T, Maruyama K, Shimada Y. Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy. Cell Tissue Res. 1998;293:305-11 pubmed
| |
L Ecuyer T, Noller J, Fulton A. Assembly of tropomyosin isoforms into the cytoskeleton of avian muscle cells. Pediatr Res. 1998;43:813-22 pubmed
| |
Muthuchamy M, Grupp I, Grupp G, O Toole B, Kier A, Boivin G, et al. Molecular and physiological effects of overexpressing striated muscle beta-tropomyosin in the adult murine heart. J Biol Chem. 1995;270:30593-603 pubmed
| |
Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
| |
Lourim D, Lin J. Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro. J Cell Biol. 1989;109:495-504 pubmed
| |
Maier A, Zak R. Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers. Histochemistry. 1990;93:423-8 pubmed
|
产品信息
内部号码 :
2988
名称 :
CH1
Depositor Name :
Lin, J.J.-C.
Depositor Institution :
University of Iowa
Date Deposited :
9/8/88
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
tropomyosin, striated muscle
抗原物种 :
鸡
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Axolotl,Broad species,Chicken,Fish,Human,Mouse,Rat,Xenopus
Species Tested (legacy) :
鸡, 大鼠, 小鼠, 蛙
Initial Publication Pubmed ID :
3899907
Collections :
Cardiac development,Cytoskeleton,Human,Muscular dystrophy,Skeletal muscle,Xenopus
搜索关键词 :
Lin, J.J.-C., tropomyosin, striated muscle, Chicken, MIgG1, chicken/rat/mouse/Xenopus/Human/Axolotl/fish/broad species, TPM1, , AB_2205770, monoclonal, Cardiac development/Muscular dystrophy/Skeletal muscle/Xenopus/Cytoskeletal elements/Human, Immunofluorescence/Western Blot/ELISA/FFPE/Function Blocking/Immunoprecipitation
抗原分子量 :
Apparent: 39 kDa (or smaller depending on the isoform)
基因 :
TPM1
UniProt数据库编号 :
P04268
抗体登记处号码 :
AB_2205770
免疫原 :
纯化鸡心脏肌肉原肌球蛋白
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
NS1
表位定位 :
Yes
Epitope Location or Sequence :
CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].
表位定位Pubmed登录号 :
24958154 22069507
Recommended Applications :
ELISA,FFPE,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
免疫荧光PubMed IDs :
15249230 9853962 26310270 25952972 27194630 27081572 25984327 24633745 2323957 9662653 10644411 16107252 15845393
免疫印迹PubMed IDs :
3899907 21088205 26171250 24958154 26041514 27553280 26447205 25984327 8530495 2668298 23712688 31411733
免疫沉淀PubMed IDs :
9621993
酶联免疫吸附测定PubMed IDs :
26024675 16981728 27429059 17260966
Function Blocking Pubmed IDs :
3899907
其他信息 :
CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.
DSHB增长培养基 :
RPMI
References (legacy) :
Hybridoma 4, 223-242.; Histochem. 93, 423-428.; J. Tiss. Cult. Meth. 14, 31-36.; Cell Tissue Res. 293, 305-311.; Dev. Biol. 218, 64-73.; FEBS Lett. 579, 4843-4850.; Biochem. Biophys. Res. Commun. 331, 303-308.
公司信息
Developmental Studies Hybridoma Bank
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。