产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗NFkB p105/p50/NFKB1抗体
目录 :
PA2111
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
![武汉博士德生物工程有限公司 PA2111 图像 1](http://www.labome.com/product/Boster/5522892_0.jpg)
Anti-NFkB p105/p50 antibody, PA2111, Western blotting. All lanes: Anti NFkB p105/p50 (PA2111) at 0.5ug/ml.
Lane 1: Rat Liver Tissue Lysate at 50ug.
Lane 2: Rat Spleen Tissue Lysate at 50ug.
Lane 3: Rat Testis Tissue Lysate at 50ug.
Lane 4: HELA Whole Cell Lysate at 40ug.
Lane 5: A431 Whole Cell Lysate at 40ug.
Lane 6: JURKAT Whole Cell Lysate at 40ug.
Predicted bind size: 50KD.
Observed bind size: 105KD
产品信息
SKU号 :
PA2111
产品名称 :
抗NFkB p105/p50/NFKB1抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 大鼠, 小鼠
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-NFkB p105/p50/NFKB1 Antibody catalog # PA2111. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
NFKB1
UniProt数据库编号 :
P25799
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NFkB p105 (778-797aa WQVFDILNGKPYEPVFTSDD), identical to the related rat sequence, and different from the related human sequence by one amino acid.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Nuclear factor NF-kappa-B p105 subunit
同义词 :
Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p50 subunit; Nfkb1;
蛋白名 :
Nuclear factor NF-kappa-B p105 subunit
分子量 :
105615分子量
Protein Function :
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis. Isoform 5, isoform 6 and isoform 7 act as inhibitors of transactivation of p50 NF- kappa-B subunit, probably by sequestering it in the cytoplasm. Isoform 3 (p98) (but not p84 or p105) acts as a transactivator of NF-kappa-B-regulated gene expression. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
Subcellular Localization :
Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Contains 7 ANK repeats.
背景 :
Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.
研究种类 :
Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Intracellular, Macrophage / Inflamm., Nfkb Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Transcription Factors
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/2506.png)
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits
微博分享
关注我们的微博
- 来邦网
- 英文来邦
问题和意见