抗体

抗腱糖蛋白R TNR抗体

PA1695-1

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化

抗腱糖蛋白R TNR抗体

315美元

100μg/vial

多克隆

兔

人类, 小鼠, 大鼠

鸡

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Rat, Human, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

WB: The detection limit for TNR is approximately 3ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Tenascin-R TNR Antibody catalog # PA1695-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

TNR

Q92752

A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNR (104-117aa QTSDHESQVTFTHK), identical to the related rat sequence, and different from the related human sequence by one amino acid.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

腱糖蛋白R

Tenascin-R; TN-R; Janusin; Restrictin; TNR;

腱糖蛋白R(TN-R)

149562分子量

Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance (By similarity).

Secreted, extracellular space, extracellular matrix.

Brain specific.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the tenascin family.

Tenascin-R is a protein that in humans is encoded by the TNR gene. Tenascin-R (TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.

Axonal Guidance Proteins, Cell Adhesion Proteins, Cytoskeleton / Ecm, Developmental Biology, Ecm Proteins, Extracellular Matrix, Growth And Development, Nervous System Development, Neurology Process, Neuroscience, Organogenesis, Signal Transduction