抗组蛋白H2B(乙酰K20)HIST1H2BB兔单克隆抗体 | 武汉博士德生物工程有限公司 P12718 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗组蛋白H2B(乙酰K20)HIST1H2BB兔单克隆抗体

P12718

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

AC-8

反应物种 :

人类, 小鼠

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

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图像

图像 1 :

Western blot analysis of Histone H2B (acetyl K20) expression in Hela cell treated with TSA lysate (P12718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H2BB monoclonal antibody (Catalog # P12718) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H2BB

产品信息

SKU号 :

P12718

产品名称 :

抗组蛋白H2B(乙酰K20)HIST1H2BB兔单克隆抗体

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

AC-8

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

应用(s) :

流式细胞仪, 免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹

应用细节 :

免疫印迹1:5000-1:20000。免疫组化1:50-1:200。免疫细胞化学/IF 1:50-1:200。免疫沉淀1:10。流式细胞仪1:10。 ChIP

描述 :

Boster Bio Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody catalog # P12718. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

HIST1H2BB

UniProt数据库编号 :

P33778

免疫原 :

A synthesized peptide derived from human Histone H2B

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

组蛋白H2B类型1-B

同义词 :

组蛋白H2B类型1-B;组蛋白H2B.1;组蛋白H2B.f;H2B/f;HIST1H2BB;H2BFF;

分子量 :

13950分子量

Protein Function :

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subcellular Localization :

Nucleus. Chromosome.

研究种类 :

ChIP’ing Antibodies, Epigenetics and Nuclear Signaling, Histones, Unmodified

更多信息或购买 :

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公司信息