Anti-Phospho-PKC zeta (T560) PRKCZ Rabbit Monoclonal Antibody | 武汉博士德生物工程有限公司 P01796 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

Anti-Phospho-PKC zeta (T560) PRKCZ Rabbit Monoclonal Antibody

P01796

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

GED-16

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 免疫组化

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图像

图像 1 :

Western blot analysis of Phospho-PKC zeta (T560) expression in HeLa cell treated with Calyculin A lysate (P01796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCZ monoclonal antibody (Catalog # P01796) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCZ

产品信息

SKU号 :

P01796

产品名称 :

Anti-Phospho-PKC zeta (T560) PRKCZ Rabbit Monoclonal Antibody

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

GED-16

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

应用(s) :

免疫组化, 免疫印迹

应用细节 :

免疫印迹1:1000-1:2000。免疫组化1:50-1:200

描述 :

Boster Bio Anti-Phospho-PKC zeta (T560) PRKCZ Rabbit Monoclonal Antibody catalog # P01796. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

PRKCZ

UniProt数据库编号 :

Q05513

免疫原 :

A synthesized peptide derived from human PKC zeta

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

Protein kinase C zeta type

同义词 :

Protein kinase C zeta type; 2.7.11.13; nPKC-zeta; PRKCZ; PKC2;

分子量 :

67660分子量

Protein Function :

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro (PubMed:17313651).

Subcellular Localization :

Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells. Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. Colocalizes with VAMP2 and WDFY2 in intracellular vesicles.

组织特异性 :

Expressed in brain, and to a lesser extent in lung, kidney and testis.

研究种类 :

Cancer, Cardiovascular, Hypertrophy, Protein Phosphorylation, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction

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