产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷酸化Hsp27(S82)HSPB1兔单克隆抗体
目录 :
P00676-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
FGD-8
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock (P00676-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1
产品信息
SKU号 :
P00676-1
产品名称 :
抗磷酸化Hsp27(S82)HSPB1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
FGD-8
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫沉淀, 免疫印迹
应用细节 :
免疫印迹1:500-1:1000。 免疫沉淀1:50
描述 :
Boster Bio Anti-Phospho-Hsp27 (S82) HSPB1 Rabbit Monoclonal Antibody catalog # P00676-1. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
HSPB1
UniProt数据库编号 :
P04792
免疫原 :
A synthesized peptide derived from human Phospho-Hsp27 (S82)
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
热休克蛋白乙1
同义词 :
Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28;
分子量 :
22783分子量
Protein Function :
Involved in stress resistance and actin organization.
Subcellular Localization :
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
组织特异性 :
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
研究种类 :
Atherosclerosis, Cancer, Cardiovascular, Chaperones, Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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