产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)
目录 :
MA1064
规格 :
100μg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
NOQ7.5。 4D
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of Myosin(Skeletal, Slow) using anti- Myosin(Skeletal, Slow) antibody (MA1064). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysates, . Lane 2: rat skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.
产品信息
SKU号 :
MA1064
产品名称 :
Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)
价格 :
315美元
规格 :
100μg/vial
克隆性 :
单克隆
克隆编号 :
NOQ7.5。 4D
宿主 :
小鼠
反应物种 :
人类, 小鼠, 兔, 大鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, mouse, rabbit, rat, By Heat.
Western blot, 0.5-2µg/ml, Human, mouse, rabbit, rat.
应用注释 :
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Myosin (Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) catalog # MA1064. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
MYH7
UniProt数据库编号 :
P12883
免疫原 :
人类骨骼肌肌球蛋白纯化来自肌原纤维
形式 :
冻干
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
腹水
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
肌球蛋白7
同义词 :
Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta; Myh7;
蛋白名 :
肌球蛋白7
分子量 :
223097分子量
Protein Function :
Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
Subcellular Localization :
Cytoplasm, myofibril. Thick filaments of the myofibrils.
组织特异性 :
MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Sequence Similarities :
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
背景 :
Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin (slow),, also known as light chain 3 (MYL3), mapped to 3p. Fodor et al. (1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.
研究种类 :
Cytoskeleton, Cytoskeleton / Ecm, Motor Proteins, Myosin, Signal Transduction
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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