产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗肌动蛋白ACTA1兔单克隆抗体
目录 :
M02014-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BE-1
反应物种 :
人类, 小鼠, 大鼠, 鸡
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
![武汉博士德生物工程有限公司 M02014-1 图像 1](http://www.labome.com/product/Boster/6027577_0.jpg)
Western blot analysis of Actin expression in (1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate (M02014-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # M02014-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1
图像 2 :
![武汉博士德生物工程有限公司 M02014-1 图像 2](http://www.labome.com/product/Boster/6027577_1.jpg)
Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody(M02014-1). ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (M02014-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
![武汉博士德生物工程有限公司 M02014-1 图像 3](http://www.labome.com/product/Boster/6027577_2.jpg)
IF analysis of immunocytochemical section of Hela cells using anti- Actin antibody (M02014-1) . Actin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Actin Antibody (M02014-1) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
产品信息
SKU号 :
M02014-1
产品名称 :
抗肌动蛋白ACTA1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BE-1
宿主 :
兔
反应物种 :
鸡, 人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, 免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:3000-1:10000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50。 流式细胞仪1:50
描述 :
Boster Bio Anti-Actin ACTA1 Rabbit Monoclonal Antibody catalog # M02014-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
ACTA1
UniProt数据库编号 :
P68133
免疫原 :
A synthesized peptide derived from human Actin
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
肌动蛋白, 甲骨骼肌
同义词 :
肌动蛋白, 甲骨骼肌;阿尔法肌动蛋白;ACTA1;ACTA;
分子量 :
42051分子量
Protein Function :
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Localization :
细胞质, 细胞骨架
研究种类 :
Actin, Actin Assembly, Actin Binding Proteins, Actin Etc, Apoptosis, Cancer, Cell Death, Cytoskeleton, Cytoskeleton / Ecm, Invasion/Microenvironment, Microfilaments, Receptors, Signal Transduction, Subcellular Markers, Tags & Cell Markers
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/2506.png)
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits