产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Integrin alpha V/ITGAV Antibody Picoband™ (monoclonal, 8B10H2)
目录 :
M01561-2
规格 :
100 µg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
8B10H2
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 M01561-2 图像 1
Figure 1. Western blot analysis of ITGAV using anti-ITGAV antibody (M01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ITGAV antigen affinity purified monoclonal antibody (Catalog # M01561-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ITGAV at approximately 130 kDa. The expected band size for ITGAV is at 116 kDa.
图像 2 :
武汉博士德生物工程有限公司 M01561-2 图像 2
Figure 2. IF analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
图像 3 :
武汉博士德生物工程有限公司 M01561-2 图像 3
Figure 3. IHC analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
产品信息
New product? :
新产品
SKU号 :
M01561-2
产品名称 :
Anti-Integrin alpha V/ITGAV Antibody Picoband™ (monoclonal, 8B10H2)
价格 :
315美元
规格 :
100 µg/vial
克隆性 :
单克隆
克隆编号 :
8B10H2
宿主 :
小鼠
反应物种 :
人类
应用(s) :
IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹, 0.25 0.5微克/毫升, 人类。 免疫组化(石蜡包埋切片), 2-5微克/毫升, 人类。 免疫细胞化学/免疫荧光, 5微克/毫升, 人类
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Integrin alpha V/ITGAV Antibody Picoband™ (monoclonal, 8B10H2) catalog # M01561-2. Tested in IF, IHC-P, ICC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
基因名称 :
ITGAV
UniProt数据库编号 :
P06756
免疫原 :
E.coli-derived human Integrin alpha V/ITGAV recombinant protein (Position: H732-D970).
形式 :
冻干
Contents :
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
纯化 :
免疫原亲和纯化
抗体亚型 :
IgG2b
储存 :
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
重组 :
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
Earlu growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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