产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Band 3 Antibody Picoband™ (monoclonal, 5G2G7)
目录 :
M01146-1
规格 :
100 µg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5G2G7
反应物种 :
人类
应用 :
免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 M01146-1 图像 1
Figure 1. Western blot analysis of Band 3 using anti-Band 3 antibody (M01146-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Band 3 antigen affinity purified monoclonal antibody (Catalog # M01146-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Band 3 at approximately 102 kDa. The expected band size for Band 3 is at 102 kDa.
图像 2 :
武汉博士德生物工程有限公司 M01146-1 图像 2
Figure 2. IHC analysis of Band 3 using anti-Band 3 antibody (M01146-1). Band 3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Band 3 Antibody (M01146-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
产品信息
New product? :
新产品
SKU号 :
M01146-1
产品名称 :
Anti-Band 3 Antibody Picoband™ (monoclonal, 5G2G7)
价格 :
315美元
规格 :
100 µg/vial
克隆性 :
单克隆
克隆编号 :
5G2G7
宿主 :
小鼠
反应物种 :
人类
应用(s) :
免疫印迹, 免疫组化-P, 流式细胞仪
应用细节 :
免疫印迹, 0.25 0.5微克/毫升, 人类。 免疫组化(石蜡包埋切片), 2-5微克/毫升, 人类。 流式细胞仪, 1-3微克/1x10^6细胞, 人类
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Band 3 Antibody Picoband™ (monoclonal, 5G2G7) catalog # M01146-1. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
基因名称 :
SLC4A1
UniProt数据库编号 :
P02730
免疫原 :
E.coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with and rat Band 3, respectively.
形式 :
冻干
Contents :
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
纯化 :
免疫原亲和纯化
抗体亚型 :
IgG2b
储存 :
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
重组 :
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
ITGA3(INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. It is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. This gene is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the α3β1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit.A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits