ELISA/assay

Mouse SLIT2 ELISA Kit PicoKine®

EK1860

96 wells/kit, with removable strips.

369美元

Mouse SLIT2 ELISA Kit PicoKine®

96 wells/kit, with removable strips.

369美元

Mouse SLIT2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Slit2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

小鼠

酶联免疫吸附测定

Slit2

Q9R1B9

Expression system for standard: NS0; Immunogen sequence: Q26-Q900

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

<50 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA)

多克隆抗体来自羊, a生物素多克隆抗体来自羊

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

slit guidance ligand 2

Slit homolog 2 protein; Slit-2; Slit homolog 2 protein N-product; Slit homolog 2 protein C-product; Slit2

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system, appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Secreted.

Expressed in developing eye, in the optic stalk, and in the ventral diencephalon.

Slit homolog 2 protein is a protein that in humans is encoded by the SLIT2 gene. It is mapped to 5; 5 B3. The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants.