domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
Anti- CD63 Picoband antibody, PB9250, Western blotting. All lanes: Anti CD63 (PB9250) at 0.5ug/ml. WB: Recombinant Human CD63 Protein 0.5ng. Predicted bind size: 26KD. Observed bind size: 26KD
Anti- CD63 Picoband antibody, PB9250, Western blotting. All lanes: Anti CD63 (PB9250) at 0.5ug/ml. WB: HELA Whole Cell Lysate at 40ug. Predicted bind size: 26KD. Observed bind size: 35KD
Anti- CD63 Picoband antibody, PB9250, IHC(P). IHC(P): Human Intestinal Cancer Tissue
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (BGG-3)
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
Western blot analysis of CD63 expression in A375 cell lysate (M01080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 monoclonal antibody (Catalog # M01080-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63
Immunohistochemical analysis of paraffin-embedded human spleen, using CD63 Antibody (M01080-1). CD63 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD63 Antibody (M01080-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
小鼠 单克隆 (MX-49.129.5)
反应物种: 人类
应用: 流式细胞仪
反应物种: 人类
应用: 流式细胞仪
小鼠 单克隆 (MEM-259)
反应物种: 人类
应用: 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
反应物种: 人类
应用: 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
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