抗S-100(乙亚单位)S100钙结合蛋白B抗体(单克隆, SH-B1)
武汉博士德生物工程有限公司
目录: MA1088
小鼠 单克隆 (SH-B1)
反应物种: 人类, 大鼠
应用: 免疫组化

Anti-S-100(beta-subunit) antibody (monoclonal), MA1088, IHC(P). IHC(P): Rat Brain Tissue Lysate
规格: 100μg/vial
价格: 315美元
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抗S100乙/S100钙结合蛋白B抗体
武汉博士德生物工程有限公司
目录: PA1303
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Anti-S100 beta antibody, PA1303, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: SMMC Cell Lysate. Lane 6: JURKAT Cell Lysate. Lane 7: COLO320 Cell Lysate
规格: 100μg/vial
价格: 315美元
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抗S100钙结合蛋白B/S100乙兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00979-1
domestic rabbit 单克隆 (BCB-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Western blot analysis of S100B expression in A375 cell lysate (M00979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100B monoclonal antibody (Catalog # M00979-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100B

Immunohistochemical analysis of paraffin-embedded rat brain, using S100B Antibody(M00979-1). S100B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-S100B Antibody (M00979-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of A375 cells using anti- S100B antibody (M00979-1) . S100B was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- S100B Antibody (M00979-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100微升
价格: 315美元
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抗蛋白S100-B S100钙结合蛋白B抗体
武汉博士德生物工程有限公司
目录: A00979
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 637美元
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抗S100乙S100钙结合蛋白B抗体
武汉博士德生物工程有限公司
目录: A00979S100
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
规格: 100微升
价格: 697美元
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抗S100钙结合蛋白B(星形胶质细胞和黑色素瘤标记)单克隆抗体
武汉博士德生物工程有限公司
目录: M00979
小鼠 单克隆 (SH-B1)
反应物种: 人类
应用: 免疫组化
规格: 100 ug/vial
价格: 440美元
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抗S100乙兔单克隆抗体, Clone#RM304
武汉博士德生物工程有限公司
目录: M00979-2
domestic rabbit 单克隆 (RM304)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 399美元
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