Anti-Bcl-XL/BCL2L1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00181
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Figure 1. Western blot analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,. Lane 2: NIH3T3 whole cell lysates,. Lane 3: HEPA1-6 whole cell lysates,. Lane 4: SGC7901 whole cell lysates,. Lane 5: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XL antigen affinity purified polyclonal antibody (Catalog # A00181) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-XL at approximately 30KD. The expected band size for Bcl-XL is at 26KD.

Figure 2. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Bcl-XL was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bcl-XL Antibody (A00181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Bcl-XL was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bcl-XL Antibody (A00181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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Anti-Bcl-XS/BCL2L1 Antibody
武汉博士德生物工程有限公司
目录: PA1232
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Anti-Bcl-XS antibody, PA1232, Western blotting. All lanes: Anti Bcl-XS (PA1232) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Predicted bind size: 19KD. Observed bind size: 19KD

Anti-Bcl-XS antibody, PA1232, IHC(P). IHC(P): Human Colon Cancer Tissue
规格: 100μg/vial
价格: 315美元
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Anti-Bcl-X/BCL2L1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9917
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Figure 1. Western blot analysis of Bcl-X using anti- Bcl-X antibody (PB9917). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Bcl-X antigen affinity purified polyclonal antibody (Catalog # PB9917) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-X at approximately 29 KD, 60KD. The expected band size for Bcl-X is at 26KD.
规格: 100μg/vial
价格: 315美元
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Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M00181-1
domestic rabbit 单克隆 (AOD-2)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Immunohistochemical analysis of paraffin-embedded human liver cancer, using Bcl-XL Antibody(M00181-1). BCL2L1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL2L1 Antibody (M00181-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Bcl-XL expression in (1) K562 cell lysate; (2) C6 cell lysate (M00181-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2L1 monoclonal antibody (Catalog # M00181-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2L1
规格: 100微升
价格: 315美元
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Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody
武汉博士德生物工程有限公司
目录: A00181-Dyl488
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪
规格: 50 ug/vial
价格: 415美元
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Anti-Human Bcl-XL DyLight® 550 conjugated BCL2L1 Antibody
武汉博士德生物工程有限公司
目录: A00181-Dyl550
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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