domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Anti-Kv1.1 potassium channel antibody, PA2296, All Western blotting. All lanes: Anti-KCNA1(PA2296) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 40ug. Lane 2: Rat Testis Tissue Lysate at 40ug. Lane 3: Rat Cardiac Muscle Tissue Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: U87 Whole Cell Lysate at 40ug. Lane 6: SHG Whole Cell Lysate at 40ug. Lane 7: NEURO Whole Cell Lysate at 40ug. Predicted bind size: 56KD. Observed bind size: 56KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Figure 1. Western blot analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA1 antigen affinity purified polyclonal antibody (Catalog # A01813-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA1 at approximately 75KD. The expected band size for KCNA1 is at 56KD.
规格: 100µg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (FFI-11)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
Western blot analysis of KCNA1 expression in Human fetal brain lysate (M01813). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA1 monoclonal antibody (Catalog # M01813) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA1
规格: 100微升
价格: 315美元
至产商
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
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