抗整合素αⅤITGAV兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01561
domestic rabbit 单克隆 (ABGC-9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Western blot analysis of Integrin alpha V expression in (1) A549 cell lysate; (2) Mouse spleen lysate; (3) Rat brain lysate (M01561). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAV monoclonal antibody (Catalog # M01561) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGAV
规格: 100微升
价格: 315美元
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抗整合素αⅤITGAV兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01561-1
domestic rabbit 单克隆 (CCE-9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Western blot analysis of integrin alpha V expression in A549 cell lysates (M01561-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAV monoclonal antibody (Catalog # M01561-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGAV
规格: 100微升
价格: 315美元
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抗整合素αⅤ(P804)ITGAV抗体
武汉博士德生物工程有限公司
目录: A01561
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 512美元
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抗整合素αⅤITGAV抗体
武汉博士德生物工程有限公司
目录: A01561-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 流式细胞仪
规格: 100微升
价格: 697美元
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Anti-Integrin alpha V/ITGAV Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01561-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
规格: 100µg/vial
价格: 315美元
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Anti-Integrin alpha V/ITGAV Antibody Picoband™ (monoclonal, 8B10H2)
武汉博士德生物工程有限公司
目录: M01561-2
小鼠 单克隆 (8B10H2)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Figure 1. Western blot analysis of ITGAV using anti-ITGAV antibody (M01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ITGAV antigen affinity purified monoclonal antibody (Catalog # M01561-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ITGAV at approximately 130 kDa. The expected band size for ITGAV is at 116 kDa.

Figure 2. IF analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 3. IHC analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
规格: 100 µg/vial
价格: 315美元
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