domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
Anti-CD11b Picoband antibody , PB9140-1.jpg. All lanes: Anti CD11B (PB9140) at 0.5ug/ml. WB: Recombinant Human CD11B Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD
Anti-CD11b Picoband antibody , PB9140-2.JPG. IHC(P): Human Tonsil Tissue
Anti-CD11b Picoband antibody , PB9140-3.JPG. IHC(P): Mouse Spleen Tissue
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates,. Lane 2: rat thymus tissue lysates,. Lane 3: mouse spleen tissue lysates,. Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # A00144-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD.
规格: 100µg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
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