抗体

抗GDNF抗体

蚂蚁-014

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

蚂蚁-014

Primary Antibody>Intracellular>Non-Labeled

Insert

抗GDNF抗体

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human GDNF. Anti-GDNF Antibody (#ANT-014) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize GDNF from human, mouse and rat samples.

A兔多克隆抗体GDNF

BLP-NT014

GDNF, Glial-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor

GDNF

Neurotrophic Factors and Receptors > Alzheimer’s Disease > Antibodies

未共轭

免疫细胞化学, IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

GDNF

人类(试验);小鼠(试验);大鼠(试验)

Mouse, dog, monkey, bovine - identical; rat 15/16 amino acid residues identical.

Glial cell-line derived neurotrophic factor (GDNF) is a neurotrophic factor originally identified for its ability to promote the survival of dopaminergic neurons in the midbrain.1 GDNF is part of the neurotrophic factor family known as GDNF-family ligand (GFL), which includes neurturin (NRTN), artemin (ARTN), and persephin (PSPN).2,3 The GFL ligands are structurally related to the transforming growth factor-b (TGF-b) protein superfamily and contain seven cysteine residues with the same relative spacing as other members of this family.2 Typical of neurotrophic factors, GDNF is produced in the form of a precursor, preproGDNF, which is then cleaved during secretion to become proGDNF, and later processed to the mature (homodimeric) GDNF form.2 The GFLs convey their activity by binding to a multicomponent receptor that includes the RET receptor tyrosine kinase and a glycosyl phosphatidylinositol (GPI)-linked ligand-binding subunit known as GDNF family receptor α (GFRα).2,3 Four different GFRα subunits have been identified (GFRα 1-4) that determine the specificity of the GFRα-RET receptor complex. Thus, GFRα1 together with RET make up the high affinity receptor for GDNF. The GDNF and GFRα complex interaction with RET induces activation of the intracellular tyrosine kinase domain of the latter. Phosphorylated RET can then interact with several intracellular signaling cascades including MAP kinase, PI3K and PLCγ signaling pathways.2,3 Lately a RET-independent signaling pathway for GDNF has been shown to involve binding of GDNF and GFRα to the neural cell adhesion molecule (NCAM). Signaling of GDNF via the NCAM complex stimulates intracellular tyrosine kinases such as Fyn and FAK.4 GDNF is synthesized and secreted by a wide variety of cell types in the central and peripheral nervous system where it exerts its neurotrophic effects in the development and maintenance of distinct sets of neurons. Accordingly, GDNF knockout mice die shortly after birth, reflecting the central role of GDNF in neurodevelopment.2  Remarkably, GDNF was found to be an essential morphogen for kidney formation as well as to have a role is spermatogenesis.3

合成肽

intracellular

C-terminus of the mature GDNF

(C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor)

Peptide (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905). C-terminus of the mature GDNF.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P39905

蚂蚁-010, AN-240, 蚂蚁-004, 蚂蚁-006, 蚂蚁-003

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元

Expression of GDNF in rat C6 glioma cells - Immunocytochemical staining of GDNF in rat C6 glioma cells. A. Paraformaldehyde-fixed and permeabilized rat C6 glioma cells were stained with Anti-GDNF Antibody (#ANT-014), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as in (A).