产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗BDNF抗体
目录 :
蚂蚁-010
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
Alomone Labs 产品网页
文章摘录数: 26
出版应用/物种/样本/稀释参考文献
  • 免疫组化; 大鼠; 1:300; 图 4di
Cohen S, Matar M, Vainer E, Zohar J, Kaplan Z, Cohen H. Significance of the orexinergic system in modulating stress-related responses in an animal model of post-traumatic stress disorder. Transl Psychiatry. 2020;10:10 pubmed 出版商
Belity T, Horowitz M, Hoffman J, Epstein Y, Bruchim Y, Todder D, et al. Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress: The Role of HSP-70 in Modulating Stress Responses. Int J Mol Sci. 2022;23: pubmed 出版商
Fyk Kolodziej B, Mueller P. Sedentary Conditions Promote Subregionally Specific Changes in Brain-Derived Neurotrophic Factor in the Rostral Ventrolateral Medulla. Front Physiol. 2021;12:756542 pubmed 出版商
Vignoli B, Sansevero G, Sasi M, Rimondini R, Blum R, Bonaldo V, et al. Astrocytic microdomains from mouse cortex gain molecular control over long-term information storage and memory retention. Commun Biol. 2021;4:1152 pubmed 出版商
Duarte Silva E, Meiry da Rocha Araújo S, Oliveira W, Lós D, Bonfanti A, Peron G, et al. Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord. Front Immunol. 2021;12:671511 pubmed 出版商
Elsayed H, Faroni A, Ashraf M, Osuji J, Wunderley L, Zhang L, et al. Development and Characterisation of an in vitro Model of Wallerian Degeneration. Front Bioeng Biotechnol. 2020;8:784 pubmed 出版商
Poddar I, Callahan P, Hernandez C, Pillai A, Yang X, Bartlett M, et al. Oral quetiapine treatment results in time-dependent alterations of recognition memory and brain-derived neurotrophic factor-related signaling molecules in the hippocampus of rats. Pharmacol Biochem Behav. 2020;197:172999 pubmed 出版商
Homa M, Loyens A, Eddarkaoui S, Faivre E, Deramecourt V, Maurage C, et al. The TMEM240 Protein, Mutated in SCA21, Is Expressed in Purkinje Cells and Synaptic Terminals. Cerebellum. 2020;19:358-369 pubmed 出版商
Perez V, Bermedo García F, Zelada D, Court F, Perez M, Fuenzalida M, et al. The p75NTR neurotrophin receptor is required to organize the mature neuromuscular synapse by regulating synaptic vesicle availability. Acta Neuropathol Commun. 2019;7:147 pubmed 出版商
Lambert W, Carlson B, Ghose P, Vest V, Yao V, Calkins D. Towards A Microbead Occlusion Model of Glaucoma for a Non-Human Primate. Sci Rep. 2019;9:11572 pubmed 出版商
Kim D, Cho J, Kang H. Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice. Behav Brain Res. 2019;374:112105 pubmed 出版商
Shin M, Choi M, Chae H, Kim J, Kim H, Kim K. Ganglioside GQ1b ameliorates cognitive impairments in an Alzheimer's disease mouse model, and causes reduction of amyloid precursor protein. Sci Rep. 2019;9:8512 pubmed 出版商
Eremenko E, Mittal K, Berner O, Kamenetsky N, Nemirovsky A, Elyahu Y, et al. BDNF-producing, amyloid β-specific CD4 T cells as targeted drug-delivery vehicles in Alzheimer's disease. EBioMedicine. 2019;43:424-434 pubmed 出版商
Escudero C, Cabeza C, Moya Alvarado G, Maloney M, Flores C, Wu C, et al. c-Jun N-terminal kinase (JNK)-dependent internalization and Rab5-dependent endocytic sorting mediate long-distance retrograde neuronal death induced by axonal BDNF-p75 signaling. Sci Rep. 2019;9:6070 pubmed 出版商
Schiller A, Hong J, Xia Z, Wang H. Increased Brain-Derived Neurotrophic Factor in Lumbar Dorsal Root Ganglia Contributes to the Enhanced Exercise Pressor Reflex in Heart Failure. Int J Mol Sci. 2019;20: pubmed 出版商
Yannai S, Zonszain J, Donyo M, Ast G. Combinatorial treatment increases IKAP levels in human cells generated from Familial Dysautonomia patients. PLoS ONE. 2019;14:e0211602 pubmed 出版商
Sun C, KRIMM R, Hill D. Maintenance of Mouse Gustatory Terminal Field Organization Is Dependent on BDNF at Adulthood. J Neurosci. 2018;38:6873-6887 pubmed 出版商
Ignowski E, Winter A, Duval N, Fleming H, Wallace T, Manning E, et al. The cysteine-rich whey protein supplement, Immunocal®, preserves brain glutathione and improves cognitive, motor, and histopathological indices of traumatic brain injury in a mouse model of controlled cortical impact. Free Radic Biol Med. 2018;124:328-341 pubmed 出版商
Yun D, Zhuang Y, Kreutz M, Behnisch T. The role of 19S proteasome associated deubiquitinases in activity-dependent hippocampal synaptic plasticity. Neuropharmacology. 2018;133:354-365 pubmed 出版商
Krizo J, Moreland L, Rastogi A, MOU X, Prosser R, Mintz E. Regulation of Locomotor activity in fed, fasted, and food-restricted mice lacking tissue-type plasminogen activator. BMC Physiol. 2018;18:2 pubmed 出版商
Dragatsis I, Dietrich P, Ren H, Deng Y, Del Mar N, Wang H, et al. Effect of early embryonic deletion of huntingtin from pyramidal neurons on the development and long-term survival of neurons in cerebral cortex and striatum. Neurobiol Dis. 2018;111:102-117 pubmed 出版商
Obradovic A, Atluri N, Dalla Massara L, Oklopcic A, Todorovic N, Katta G, et al. Early Exposure to Ketamine Impairs Axonal Pruning in Developing Mouse Hippocampus. Mol Neurobiol. 2018;55:164-172 pubmed 出版商
Fukuchi M, Izumi H, Mori H, Kiyama M, Otsuka S, Maki S, et al. Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice. Sci Rep. 2017;7:4949 pubmed 出版商
Hoffman J, Zuckerman A, Ram O, Sadot O, Stout J, Ostfeld I, et al. Behavioral and inflammatory response in animals exposed to a low-pressure blast wave and supplemented with β-alanine. Amino Acids. 2017;49:871-886 pubmed 出版商
Benhar I, Reemst K, Kalchenko V, Schwartz M. The retinal pigment epithelium as a gateway for monocyte trafficking into the eye. EMBO J. 2016;35:1219-35 pubmed 出版商
Wattananit S, Tornero D, Graubardt N, Memanishvili T, Monni E, Tatarishvili J, et al. Monocyte-Derived Macrophages Contribute to Spontaneous Long-Term Functional Recovery after Stroke in Mice. J Neurosci. 2016;36:4182-95 pubmed 出版商
图像
图像 1 :
Alomone Labs ANT-010 图像 1
Western blot analysis with Anti-BDNF Antibody: - 1,5. Recombinant human BDNF protein (#B-250).2,6. Recombinant mouse proBDNF protein (#B-240).3,7. Recombinant human beta-NGF protein (#N-245).4,8. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260).Lanes 1-4: Anti-BDNF Antibody (#ANT-010), (1:200).Lanes 5-8: Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010). Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.
图像 2 :
Alomone Labs ANT-010 图像 2
Expression of BDNF in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-BDNF Antibody (#ANT-010). A. BDNF (red) appears in Purkinje cells (upward pointing arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (downward pointing arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein (green) in the same section demonstrates the distribution of BDNF to neuronal as well as to astrocytic cellular components. C. Confocal merge of BDNF and GFAP.
图像 3 :
Alomone Labs ANT-010 图像 3
Peptide (C)GEFGGHDKPKITAWE corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (AccessionO35458). Cytoplasmic N-terminus.
产品信息
SKU号 :
蚂蚁-010_1_25_mcl
ParentSKU :
蚂蚁-010
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗BDNF抗体
产品描述 :
Anti-BDNF Antibody (#ANT-010) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human, and mouse samples. The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4.
Short Description :
A兔多克隆抗体BDNF
Negative Control :
BLP-NT010
Positive Control :
PCP-B25
同义词 :
脑源性神经营养因子, 脑源性神经营养因子
靶标名 :
BDNF
Primary Category (Old) :
Neurotrophic Factors and Receptors > Neurotrophins > Antibodies
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, 免疫组化, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
BDNF
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse, rat and many other species - identical.
靶标/背景信息 :
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system. Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its “rapid” neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.
免疫原 :
合成肽
区域 :
intracellular
Loop :
Intracellular
序列 :
(C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)
序列文本 :
Peptide (C)VLEKVPVSKGQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor) (Accession P23560).
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.72毫克/毫升
浓度 :
072毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P23560
Top Related :
蚂蚁-006, B-250, AN-240, AGP-021, 蚂蚁-019
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
更多信息或购买 :
Alomone Labs 产品网页
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。