抗体

豚鼠抗BDNF抗体

蚂蚁-010

1 X 25 mcl

449美元

多克隆

豚鼠

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

蚂蚁-010

Primary Antibody>Intracellular>Non-Labeled

Insert

豚鼠抗BDNF抗体

Guinea pig Anti-BDNF Antibody (#ANT-010-GP) is a highly specific antibody directed against an epitope of human brain-derived neurotrophic factor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human and mouse samples. The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4. The antigen used to immunize guinea pigs is the same as Anti-BDNF Antibody (#ANT-010) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

A豚鼠多克隆抗体BDNF

BLP-NT010

PCP-B25

BDNF, 脑源性神经营养因子

BDNF

未共轭

IF, 免疫组化, 免疫印迹

豚鼠

多克隆

豚鼠总数IgG

BDNF

人类(试验);小鼠(试验);大鼠(试验)

Mouse, rat and many other species - identical.

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system. Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its “rapid” neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

合成肽

intracellular

Intracellular

(C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)

Peptide (C)VLEKVPVSKGQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor) (Accession P23560).

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P23560

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

449美元