产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗突触素抗体
目录 :
ANR-013
规格 :
1 X 50 mcl
价格 :
530美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 2
出版应用/物种/样本/稀释 | 参考文献 |
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| |
图像
图像 1 :
![Alomone Labs ANR-013 图像 1](http://www.labome.com/product/Alomone-Labs/5482409_0.gif)
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat PC12 pheochromocytoma cell line lysate (lanes 3 and 6): - 1-3. Anti-Synaptophysin Antibody (#ANR-013), (1:400).4-6. Anti-Synaptophysin Antibody, preincubated with Synaptophysin Blocking Peptide (#BLP-NR013).
图像 2 :
![Alomone Labs ANR-013 图像 2](http://www.labome.com/product/Alomone-Labs/5482409_1.jpg)
Expression of Synaptophysin in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Synaptophysin Antibody (#ANR-013), (1:100). SYP (red) is expressed in DRG neurons. Hoechst 33342 (blue) shows nuclear staining and is used as the counterstain.
图像 3 :
![Alomone Labs ANR-013 图像 3](http://www.labome.com/product/Alomone-Labs/5482409_2.jpg)
Expression of Synaptophysin in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections usingAnti-SynaptophysinAntibody (#ANR-013) (1:100). SYP (red) is expressed in DRG neurons. Hoechst 33342 (blue) shows nuclear staining and is used as the counterstain.
产品信息
SKU号 :
ANR-013_1_50_mcl
ParentSKU :
ANR-013
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗突触素抗体
产品描述 :
Anti-Synaptophysin Antibody (#ANR-013) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize synaptophysin from mouse, rat, and human samples.
Short Description :
A兔多克隆抗体突触素
Negative Control :
BLP-NR013
同义词 :
Synaptophysin, Major synaptic vesicle protein p38, SYP
靶标名 :
突触素
Primary Category (Old) :
Neural Signaling > Exocytotic Machinery Proteins > Antibodies
标记/共轭标签 :
未共轭
应用 :
IF, 免疫组化, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
SYP
反应物种 :
Mouse (Tested);Rat (Tested);Human (Predicted)
同源 :
Rat, mouse, human - 12/13 amino acid residues identical.
靶标/背景信息 :
Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.
Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.
Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.
Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.
免疫原 :
合成肽
区域 :
intracellular
Loop :
2nd intravesicular loop
序列 :
CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin
序列文本 :
Peptide CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin (Accession P07825). 2nd intravesicular loop.
Primary Size (Size+Units) :
1 X 50 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P07825
Top Related :
ANR-014, APZ-009, ANR-003, ANR-113, ANR-111
储存和重组 :
Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
50
单位 :
mcl
标价美国[美元] :
530美元
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/78.gif)
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。