抗体

豚鼠抗突触素抗体

ANR-013-GP

1 X 0.2毫升

673美元

多克隆

豚鼠

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化

ANR-013-GP

Primary Antibody>Intracellular>Non-Labeled

Insert

豚鼠抗突触素抗体

Guinea Pig Anti-Synaptophysin Antibody (#ANR-013-GP) is a highly specific antibody directed against an epitope of the rat Synaptophysin protein. The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize Synaptophysin from rat, mouse and human samples. n nThe antigen used to immunize guinea pigs is the same as Anti-Synaptophysin Antibody (#ANR-013) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

A豚鼠多克隆抗体突触素

BLP-NR013

Major synaptic vesicle protein p38, SYP

突触素

未共轭

免疫组化, 免疫印迹

豚鼠

多克隆

豚鼠总数IgG

SYP

人类(试验);小鼠(试验);大鼠(试验)

Rat, mouse, human – 12 out of 13 amino acid residues identical.

Protease-activated receptor 4 (PAR-4) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3 Different PARs are activated by different proteases. Hence, PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated only by thrombin and PAR-2 is activated only by trypsin.1-3 PAR-4 can be also cleaved and activated by other proteases such as cathepsin G. The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they involve calcium mobilization downstream of phospholipase Cβ through the Gαq pathway.1-3 Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived from PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation.PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function.1-3

合成肽

intracellular

intravesicular loop

CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat Synaptophysin

Peptide CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat Synaptophysin (Accession P07825). 2nd intravesicular loop.

1 X 0.2毫升

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P07825

Reconstitution: 1 X 0.2 ml double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

0.2

毫升

673美元