产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
豚鼠抗突触素抗体
目录 :
ANR-013-GP
规格 :
1 X 0.2毫升
价格 :
673美元
克隆性 :
多克隆
宿主 :
豚鼠
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
![Alomone Labs ANR-013-GP 图像 1](http://www.labome.com/product/Alomone-Labs/6380754_0.jpg)
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Guinea Pig Anti-Synaptophysin Antibody (#ANR-013-GP), (1:200). 3-4. Guinea Pig Anti-Synaptophysin Antibody, preincubated with Synaptophysin Blocking Peptide (BLP-NR013).
图像 2 :
![Alomone Labs ANR-013-GP 图像 2](http://www.labome.com/product/Alomone-Labs/6380754_1.jpg)
Expression of Synaptophysin in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-Synaptophysin Antibody (#ANR-013-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. Staining in the hippocampal CA3 region, showed immunoreactivity (red) in the mossy fiber terminal zone (MF, arrows). B. Pre-incubation of the antibody with Synaptophysin Blocking Peptide (BLP-NR013), suppressed staining. Cell nuclei are stained with DAPI (blue).
产品信息
SKU号 :
ANR-013-GP_1_0.2_ml
ParentSKU :
ANR-013-GP
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
豚鼠抗突触素抗体
产品描述 :
Guinea Pig Anti-Synaptophysin Antibody (#ANR-013-GP) is a highly specific antibody directed against an epitope of the rat Synaptophysin protein. The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize Synaptophysin from rat, mouse and human samples.
n
nThe antigen used to immunize guinea pigs is the same as Anti-Synaptophysin Antibody (#ANR-013) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
Short Description :
A豚鼠多克隆抗体突触素
Negative Control :
BLP-NR013
同义词 :
Major synaptic vesicle protein p38, SYP
靶标名 :
突触素
标记/共轭标签 :
未共轭
应用 :
免疫组化, 免疫印迹
宿主 :
豚鼠
克隆性 :
多克隆
抗体亚型 :
豚鼠总数IgG
基因符号 :
SYP
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Rat, mouse, human – 12 out of 13 amino acid residues identical.
靶标/背景信息 :
Protease-activated receptor 4 (PAR-4) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3
Different PARs are activated by different proteases. Hence, PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated only by thrombin and PAR-2 is activated only by trypsin.1-3 PAR-4 can be also cleaved and activated by other proteases such as cathepsin G.
The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they involve calcium mobilization downstream of phospholipase Cβ through the Gαq pathway.1-3
Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived from PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation.PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function.1-3
免疫原 :
合成肽
区域 :
intracellular
Loop :
intravesicular loop
序列 :
CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat Synaptophysin
序列文本 :
Peptide CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat Synaptophysin (Accession P07825). 2nd intravesicular loop.
Primary Size (Size+Units) :
1 X 0.2毫升
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P07825
储存和重组 :
Reconstitution: 1 X 0.2 ml double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
0.2
单位 :
毫升
标价美国[美元] :
673美元
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/78.gif)
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。