pLuc-CDS-m640-1+2 | Addgene 42106 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pLuc-CDS-m640-1+2

42106

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Mazda M, Nishi K, Naito Y, Ui Tei K. E-cadherin is transcriptionally activated via suppression of ZEB1 transcriptional repressor by small RNA-mediated gene silencing. PLoS ONE. 2011;6:e28688 pubmed 出版商

产品信息

目录号 :

42106

产品名称 :

pLuc-CDS-m640-1+2

文章 :

doi	10.1371/journal.pone.0028688
id	6171
pubmed_id	22205962

细菌的抗性 :

氨苄西林

克隆 :

backbone

psiCHECK-1

backbone_mutation

The stop codon of Renilla luciferase was removed, and a SalI restriction site was added by PCR.

backbone_origin

Promega

backbone_size

3565

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

起源 :

主要研究者 :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SalI
promoter	SV40
sequencing_primer_3
sequencing_primer_5	CGCAACTACAACGCCTACCT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
gene	ZEB1
id	6935

genbank_ids

NM_030751.4

mutation

Silent mutations were introduced in two seed-complementary sites of dsEcad640 antisense strand (640a-1 and 640a-2).

name

ZEB1

shRNA_sequence

size

species

9606

Homo sapiens