pGL3报告载体-1574/+120 | Addgene 35539 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pGL3报告载体-1574/+120

35539

更多信息或购买 :

Addgene 产品网页

文章摘录数: 2

参考文献
Lu Z, Jiao D, Qiao J, Yang S, Yan M, Cui S, et al. Restin suppressed epithelial-mesenchymal transition and tumor metastasis in breast cancer cells through upregulating mir-200a/b expression via association with p73. Mol Cancer. 2015;14:102 pubmed 出版商
Bracken C, Gregory P, Kolesnikoff N, Bert A, Wang J, Shannon M, et al. A double-negative feedback loop between ZEB1-SIP1 and the microRNA-200 family regulates epithelial-mesenchymal transition. Cancer Res. 2008;68:7846-54 pubmed 出版商

产品信息

目录号 :

35539

产品名称 :

pGL3报告载体-1574/+120

文章 :

doi	10.1158/0008-5472.CAN-08-1942
id	5146
pubmed_id	18829540

细菌的抗性 :

氨苄西林

克隆 :

backbone

pGL3-basic

backbone_mutation

backbone_origin

Promega

backbone_size

4818

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Luciferase

生长记录 :

Please note that Addgene's sequencing results identified a few nucleotide mismatches at bp# 253, 457, 465, 632, 681 & 1463 when compared to the sequence provided by the depositing laboratory. These differences are not known to affect the function of the plasmid.

起源 :

主要研究者 :

alt_names

MIRN200B

MIRN200A

MIRN429

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglI
cloning_site_5	NheI
promoter	none
sequencing_primer_3	CTTTATGTTTTTGGCGTCTTCCA
sequencing_primer_5	-CTAGCAAAATAGGCTGTCCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIRN200B, mir-200b
gene	MIR200B
id	406984

aliases	MIRN429, hsa-mir-429, mir-429
gene	MIR429
id	554210

aliases	MIRN200A, mir-200a
gene	MIR200A
id	406983

genbank_ids

406984

406983

mutation

name

promoter region of miR200b-200a-429 genomic cluster

shRNA_sequence

size

1694

species

9606

Homo sapiens