pCI-neo-RL-ZEB1 200bmutx5 | Addgene 35537 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pCI-neo-RL-ZEB1 200bmutx5

35537

更多信息或购买 :

Addgene 产品网页

文章摘录数: 2

参考文献
Ram xed rez Moya J, Baker A, Slack F, Santisteban P. ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity. Oncogene. 2020;39:3738-3753 pubmed 出版商
Gregory P, Bert A, Paterson E, Barry S, Tsykin A, Farshid G, et al. The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Nat Cell Biol. 2008;10:593-601 pubmed 出版商

产品信息

目录号 :

35537

产品名称 :

pCI-neo-RL-ZEB1 200bmutx5

文章 :

doi	10.1038/ncb1722
id	5145
pubmed_id	18376396

细菌的抗性 :

氨苄西林

克隆 :

backbone

pCI-neo-RL

backbone_mutation

RL was inserted into pCI-neo (Promega) to form the plasmid pcI-neo-RL by Witold Filipowicz

backbone_origin

Witold Filipowicz

backbone_size

6380

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

生长记录 :

Mutations in the miR-200b seed regions of the ZEB1 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit. The ZEB1 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.

起源 :

主要研究者 :

alt_names

BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	XbaI
promoter
sequencing_primer_3	unknown
sequencing_primer_5	unknown
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
gene	ZEB1
id	6935

genbank_ids

6935

mutation

5 mutations in miR200b seed regions of 3'UTR insert

name

3'UTR of ZEB1

shRNA_sequence

size

1764

species

9606

Homo sapiens

4932

Saccharomyces cerevisiae