产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
UbC NLS-HA-MCP-YFP
目录 :
31230
更多信息或购买 :
Addgene 产品网页
文章摘录数: 6
参考文献
Guo Y, Lee R. Long-term imaging of individual mRNA molecules in living cells. Cell Rep Methods. 2022;2:100226 pubmed 出版商
Reinkemeier C, Lemke E. Condensed, Microtubule-coating Thin Organelles for Orthogonal Translation in Mammalian Cells. J Mol Biol. 2022;434:167454 pubmed 出版商
Pichon X, Moissoglu K, Coleno E, Wang T, Imbert A, Robert M, et al. The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions. RNA. 2021;27:1528-1544 pubmed 出版商
Reinkemeier C, Lemke E. Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation. Cell. 2021;184:4886-4903.e21 pubmed 出版商
Wang S, Su J, Zhang F, Zhuang X. An RNA-aptamer-based two-color CRISPR labeling system. Sci Rep. 2016;6:26857 pubmed 出版商
GRUNWALD D, Singer R. In vivo imaging of labelled endogenous ?-actin mRNA during nucleocytoplasmic transport. Nature. 2010;467:604-7 pubmed 出版商
产品信息
目录号 :
31230
产品名称 :
UbC NLS-HA-MCP-YFP
文章 :
doi10.1038/nature09438
id4369
pubmed_id20844488
细菌的抗性 :
氨苄西林
克隆 :
backbonepHAGE
backbone_mutation
backbone_origin
backbone_size6247
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
生长记录 :
Additional reference: Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA, Park HY, de Turris V, Lopez-Jones M, Singer RH. A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods. Feb;8(2):165-70 (2011)
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5NotI
promoter
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameNLS-HA-MCP-YFP
shRNA_sequence
size1171
species
artificial
tags
质粒拷贝数 :
The phage-UbC RIG vector for lentiviral expression was a gift from G. Mostoslavsky and G. Vainer (Harvard University). Mostoslavsky, G., Fabian, A.J., Rooney, S., Alt, F.W. & Mulligan, R.C. Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer. Proc. Natl. Acad. Sci. USA 103, 16406 16411 (2006).
抗性标记物 :
880
标签 :
未知
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国