pDEST-HemmarG | Addgene 31221 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pDEST-HemmarG

31221

更多信息或购买 :

Addgene 产品网页

文章摘录数: 3

参考文献
Arguello J, Abuin L, Armida J, Mika K, Chai P, Benton R. Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants. elife. 2021;10: pubmed 出版商
Prieto Godino L, Rytz R, Cruchet S, Bargeton B, Abuin L, Silbering A, et al. Evolution of Acid-Sensing Olfactory Circuits in Drosophilids. Neuron. 2017;93:661-676.e6 pubmed 出版商
Han C, Jan L, Jan Y. Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal neuron-glia interactions in Drosophila. Proc Natl Acad Sci U S A. 2011;108:9673-8 pubmed 出版商

产品信息

目录号 :

31221

产品名称 :

pDEST-HemmarG

文章 :

doi	10.1073/pnas.1106386108
id	4365
pubmed_id	21606367

细菌的抗性 :

氯霉素和氨苄西林

克隆 :

backbone

pAPIC-HIH

backbone_mutation

backbone_origin

backbone_size

10193

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

生长记录 :

Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdGFP The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog)

生长温度 :

ccdB Survival 2 T1R Cells @ 37C.

起源 :

主要研究者 :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	XhoI
promoter
sequencing_primer_3	GGCGCACAGAAATGATTACAAC
sequencing_primer_5	ACTGCAACTACTGAAATCTGCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD4mut, IMD79, Leu-3, OKT4D, T4
gene	CD4
id	920

genbank_ids

mutation

Fusion of signal peptide of Drosophila Akh gene, human CD4 transmembrane domain, EGFP, GFP, and Kir2.1 ER exit signal.

name

CD4-tdGFP

shRNA_sequence

size

2340

species

9606

Homo sapiens

7227

Drosophila melanogaster

Aequorea victoria