peCALWY-2 | Addgene 22234 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

peCALWY-2

22234

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Vinkenborg J, Nicolson T, Bellomo E, Koay M, Rutter G, Merkx M. Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis. Nat Methods. 2009;6:737-40 pubmed 出版商

产品信息

目录号 :

22234

产品名称 :

peCALWY-2

文章 :

doi	10.1038/nmeth.1368
id	2899
pubmed_id	19718032

细菌的抗性 :

卡那霉素

克隆 :

backbone

pCMV (Clontech)

backbone_mutation

backbone_origin

backbone_size

3900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

生长温度 :

E.coli nova blue LB culture medium 30 ug/ml kanamycin Culture overnight, 37 C/250 rpm

起源 :

主要研究者 :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NheI
promoter
sequencing_primer_3	CMV terminator
sequencing_primer_5	CMV promotor
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATX1, HAH1
gene	ATOX1
id	475

genbank_ids

mutation

sequence identical to eCALWY-1, but linkerlength is 5 GGSGGS repeats instead of 9

name

eCALWY-2 Zn(II) sensor

shRNA_sequence

size

2100

species

9606

Homo sapiens

GFP's