产品简要
文章摘录数: 2
参考文献 |
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Phelan M, Sif S, Narlikar G, Kingston R. Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Mol Cell. 1999;3:247-53 pubmed
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产品信息
目录号 :
1954
产品名称 :
pFastBac1 FLAG标签BAF155 b
文章 :
doi | 10.1016/S1097-2765(00)80315-9 |
id | 143 |
pubmed_id | 10078207 |
细菌的抗性 :
氨苄西林
克隆 :
backbone | pFastBac1 | |
backbone_mutation | ||
backbone_origin | Invitrogen | |
backbone_size | 4775 | |
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
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生长记录 :
We constructed a composite full-length cDNA for hSWI3 (BAF155) using a 2.4 kb partial human cDNA that was isolated as described previously and a 1.8 kb RT-PCR-amplified fragment obtained from human liver poly(A) RNA. We consistently isolated the partial hSWI3 cDNA (2.4 kb) from several libraries. It appears that an adenine-rich stretch at the 3' end of these cDNAs hybridizes with the oligo(dt) primers used to create the first strand of the cDNA. An oligo that anneals in the sense direction to the partial cDNA, and an oligo that anneals to the 3' UTR of the highly related hSWI3 clone described previously, BAF155, were used to generate the missing coding sequences. The amplified fragment was inserted into the BamHI and SpeI sites of pBS-5'hSWI3, which contains the partial hSWI3 cDNA. The protein encoded by this complete ORF is highly related to BAF155, except for a few positions scattered throughout the protein. Most of these differences are substitutions of BAF155 sequences with the corresponding sequences of BAF170. To generate pFastBac1-BAF155, an XhoI
起源 :
37
主要研究者 :
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抗性标记物 :
254 |
标签 :
High Copy
条款 :
Gentamicin |
更多信息或购买 :
公司信息
Addgene
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Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
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