产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
Punc-4c.TIR1
目录 :
194053
更多信息或购买 :
Addgene 产品网页
文章摘录数: 1
参考文献
Kurashina M, Mizumoto K. Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans. STAR Protoc. 2023;4:102028 pubmed 出版商
产品信息
目录号 :
194053
产品名称 :
Punc-4c.TIR1
文章 :
doi10.1016/j.xpro.2022.102028
id28233232
pubmed_id36640369
细菌的抗性 :
氨苄西林
克隆 :
backbonedelta-pSM
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
生长株 :
TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5AscI
promoterunc-4c
sequencing_primer_3unc-54 utr R cttagaagtcagaggcacgg
sequencing_primer_5unc-4c F GAAGTCTAGCAGGATTAAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1
geneTIR1
id825473
genbank_ids
mutationcodon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated; Derived from pLZ31 (Addgene Plasmid #71720)
nameTIR1
shRNA_sequence
size1887
species
3702
Arabidopsis thaliana
tags
质粒拷贝数 :
The TIR1 sequence was PCR-amplified from pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR) (Plasmid #71720).
抗性标记物 :
4144
标签 :
High Copy
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国