产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pBPGw
目录 :
17574
更多信息或购买 :
Addgene 产品网页
文章摘录数: 5
参考文献
Bedont J, Toda H, Shi M, Park C, Quake C, Stein C, et al. Short and long sleeping mutants reveal links between sleep and macroautophagy. elife. 2021;10: pubmed 出版商
Ahmed O, Avila Herrera A, Tun K, Serpa P, Peng J, Parthasarathy S, et al. Evolution of Mechanisms that Control Mating in Drosophila Males. Cell Rep. 2019;27:2527-2536.e4 pubmed 出版商
Lin S, Dikler S, Blincoe W, Ferguson R, Sheridan R, Peng Z, et al. Mapping the dark space of chemical reactions with extended nanomole synthesis and MALDI-TOF MS. Science. 2018;361: pubmed 出版商
Janssens D, Hamm D, Anhezini L, Xiao Q, Siller K, Siegrist S, et al. An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and Rapid Restriction of Developmental Potential during Asymmetric Stem Cell Division. Dev Cell. 2017;40:367-380.e7 pubmed 出版商
Pfeiffer B, Jenett A, Hammonds A, Ngo T, Misra S, Murphy C, et al. Tools for neuroanatomy and neurogenetics in Drosophila. Proc Natl Acad Sci U S A. 2008;105:9715-20 pubmed 出版商
产品信息
目录号 :
17574
产品名称 :
pBPGw
文章 :
doi10.1073/pnas.0803697105
id1972
pubmed_id18621688
细菌的抗性 :
氯霉素和氨苄西林
克隆 :
backbonepBDP
backbone_mutation
backbone_origin
backbone_size8902
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
生长记录 :
pBPGw is a modular Gateway compatible promoter-less GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest.
生长温度 :
Bacterial Strain DB3.1 (Invitrogen) or similar must be used to propagate plasmids carrying the ccdB gene.
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3BDP R (bp 5,286) : ATAATGGTGCAGGGCGCTGAC
sequencing_primer_5BDP F (bp 11,503): AAATAGGGGTTCCGCGCACAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL248C, GAL81
geneGAL4
id855828
genbank_ids
mutation
nameGAL4
shRNA_sequence
size2646
species
4932
Saccharomyces cerevisiae
tags
质粒拷贝数 :
GAL4 leader, CDS, and hsp70 polyA were cloned from pGaTB (via DGRC).
抗性标记物 :
428
标签 :
High Copy
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国