产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
BW-Para
目录 :
172602
更多信息或购买 :
Addgene 产品网页
文章摘录数: 1
参考文献
Picard H, Schwingen K, Green L, Shis D, Egan S, Bennett M, et al. Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes. Proteins. 2022;90:186-199 pubmed 出版商
产品信息
目录号 :
172602
产品名称 :
BW-Para
文章 :
doi10.1002/prot.26206
id28216962
pubmed_id34369028
克隆 :
backbonen/a
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
生长记录 :
The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays (after growing transformed cells on MOPS media). Plasmids encoding these chimeras are found at https://www.addgene.org/browse/article/28216962/. The protocol for the reporter assay is detailed in the manuscript. The genotype for BW-Para is [F-, (araD-araB)567, lacZ4787(::rrnB-3), -, rph-1, (rhaD-rhaB)568, hsdR514], lacI785::kan, araC771::kan, rhaSR::(PBADmut:lacZ)]. The PBADmut:lacZ reporter construct integrated into the rhaSR KO locus comprises a synthetic Para-I promoter with -10/-35 sites of GATACT/TTTACA respectively and an ara-I DNA binding site proximal to the -35 site. The integrated 3.5 kb cassette coding for the reporter construct can be amplified from the genome using the primers: (forward) 5' - GGTGAAAGTTGGAACCTCTTAC - 3' and (reverse) 5'- GCGAGGAAGCGGAATATATCCCC - 3'. Cells should be freshly transformed prior to each assay.
生长株 :
The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.
插入 :
BW-Para
起源 :
37
抗性标记物 :
3363
标签 :
未知
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国