pGRG36 | Addgene 16666 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pGRG36

16666

更多信息或购买 :

Addgene 产品网页

文章摘录数: 5

参考文献
Schlechter R, Jun H, Bernach M, Oso S, Boyd E, Muñoz Lintz D, et al. Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria. Front Microbiol. 2018;9:3052 pubmed 出版商
Lee J, Jeong E, Lee J, Jung M, Shin E, Kim Y, et al. Directed evolution of CRISPR-Cas9 to increase its specificity. Nat Commun. 2018;9:3048 pubmed 出版商
Martins R, Maier J, Gorki A, Huber K, Sharif O, Starkl P, et al. Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Nat Immunol. 2016;17:1361-1372 pubmed 出版商
Remus Emsermann M, Gisler P, Drissner D. MiniTn7-transposon delivery vectors for inducible or constitutive fluorescent protein expression in Enterobacteriaceae. FEMS Microbiol Lett. 2016;363: pubmed 出版商
McKenzie G, Craig N. Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. BMC Microbiol. 2006;6:39 pubmed

产品信息

目录号 :

16666

产品名称 :

pGRG36

文章 :

doi	10.1186/1471-2180-6-39
id	1884
pubmed_id	16646962

细菌的抗性 :

氨苄西林

克隆 :

backbone

pGRG36

backbone_mutation

backbone_origin

backbone_size

12549

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

生长记录 :

Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.

生长温度 :

DH5alpha in LB + ampicillin, grow at less than 32 degrees C

起源 :

主要研究者 :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI, AvrII, NotI, PacI, and XhoI
cloning_site_5	SmaI, AvrII, NotI, PacI, and XhoI
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

DQ460223

mutation

name

mTn7::MCS

shRNA_sequence

size

species