产品简要
文章摘录数: 5
参考文献 |
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McKenzie G, Craig N. Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. BMC Microbiol. 2006;6:39 pubmed
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产品信息
目录号 :
16666
产品名称 :
pGRG36
文章 :
doi | 10.1186/1471-2180-6-39 |
id | 1884 |
pubmed_id | 16646962 |
细菌的抗性 :
氨苄西林
克隆 :
backbone | pGRG36 | |
backbone_mutation | ||
backbone_origin | ||
backbone_size | 12549 | |
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
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生长记录 :
Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
生长温度 :
DH5alpha in LB + ampicillin, grow at less than 32 degrees C
起源 :
30
主要研究者 :
|
抗性标记物 :
98 |
标签 :
Low Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国