产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pcDNA3质粒-sPDGFRalpha
目录 :
136440
更多信息或购买 :
Addgene 产品网页
文章摘录数: 1
参考文献
Park J, Gill K, Aghajani A, Heredia J, Choi H, Oberstein A, et al. Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. PLoS Pathog. 2020;16:e1008647 pubmed 出版商
产品信息
目录号 :
136440
产品名称 :
pcDNA3质粒-sPDGFRalpha
文章 :
doi10.1371/journal.ppat.1008647
id28207324
pubmed_id32559251
细菌的抗性 :
氨苄西林
克隆 :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5332
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
生长株 :
Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)
起源 :
37
主要研究者 :
alt_names
PDGFRA
platelet-derived growth factor receptor alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD140A, PDGFR-2, PDGFR2
genePDGFRA
id5156
genbank_ids
NP_006197.1
mutationExtracellular domain of PDGFR-alpha (a.a. 1-524) with V242K mutation. Binds HCMV trimer, but no longer binds PDGFs.
namePDGFRalpha
shRNA_sequence
size2289
species
9606
Homo sapiens
tags
locationC terminal on insert
tagLinker GGGS
locationC terminal on insert
tagFc region of IgG1 (a.a. Asp-221 to Lys-447)
抗性标记物 :
3492
标签 :
High Copy
条款 :
Neomycin (select with G418)
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国