MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

131461

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Kim G, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra Clarke M, et al. Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Cell. 2019;179:251-267.e24 pubmed 出版商

产品信息

目录号 :

131461

产品名称 :

MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

文章 :

doi	10.1016/j.cell.2019.08.013
id	28203812
pubmed_id	31539496

细菌的抗性 :

氨苄西林

克隆 :

backbone

MADR pDonor

backbone_mutation

backbone_origin

Breunig Lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

Synthetic Biology

Other

Mosaic analysis for dual recombinase-mediated cassette exchange (MADR) donor

生长株 :

MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

起源 :

主要研究者 :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	none (4x polyA to mitigate episomal expression)
sequencing_primer_3	WPRE-R (addgene)
sequencing_primer_5	MADR-MCSF (TCGACCTGCAGCCCAAGCTA)
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

Pdgfra D842V

name

H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

shRNA_sequence

size

species

9606

Homo sapiens

10090

Mus musculus