产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
CROP-seq-opti
目录 :
106280
更多信息或购买 :
文章摘录数: 8
参考文献
McFaline Figueroa J, Srivatsan S, Hill A, Gasperini M, Jackson D, Saunders L, et al. Multiplex single-cell chemical genomics reveals the kinase dependence of the response to targeted therapy. bioRxiv. 2023;: pubmed 出版商
Yao D, Binan L, Bezney J, Simonton B, Freedman J, Frangieh C, et al. Compressed Perturb-seq: highly efficient screens for regulatory circuits using random composite perturbations. bioRxiv. 2023;: pubmed 出版商
Danziger O, Patel R, DeGrace E, Rosen M, Rosenberg B. Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor. PLoS Pathog. 2022;18:e1010464 pubmed 出版商
Nasser J, Bergman D, Fulco C, Guckelberger P, Doughty B, Patwardhan T, et al. Genome-wide enhancer maps link risk variants to disease genes. Nature. 2021;593:238-243 pubmed 出版商
Dhimolea E, de Matos Simoes R, Kansara D, Al Khafaji A, Bouyssou J, Weng X, et al. An Embryonic Diapause-like Adaptation with Suppressed Myc Activity Enables Tumor Treatment Persistence. Cancer Cell. 2021;39:240-256.e11 pubmed 出版商
Song M, Yang X, Ren X, Maliskova L, Li B, Jones I, et al. Mapping cis-regulatory chromatin contacts in neural cells links neuropsychiatric disorder risk variants to target genes. Nat Genet. 2019;51:1252-1262 pubmed 出版商
Gasperini M, Hill A, McFaline Figueroa J, Martin B, Kim S, Zhang M, et al. A Genome-wide Framework for Mapping Gene Regulation via Cellular Genetic Screens. Cell. 2019;176:377-390.e19 pubmed 出版商
Hill A, McFaline Figueroa J, Starita L, Gasperini M, Matreyek K, Packer J, et al. On the design of CRISPR-based single-cell molecular screens. Nat Methods. 2018;15:271-274 pubmed 出版商
产品信息
目录号 :
106280
产品名称 :
CROP-seq-opti
文章 :
doi10.1038/nmeth.4604
id28192603
pubmed_id29457792
细菌的抗性 :
氨苄西林
克隆 :
backbonelentiGuide-puro
backbone_mutation
backbone_originFeng Zhang Lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
生长株 :
A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
起源 :
30
主要研究者 :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterU6
sequencing_primer_3
sequencing_primer_5TTGGGCACTGACAATTCCGT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameEF1a-Puro-WPRE-hU6-gRNA
shRNA_sequence
size
species
100
Synthetic
tags
抗性标记物 :
2142
标签 :
High Copy
条款 :
Puromycin
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国